IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v400y1999i6740d10.1038_22133.html
   My bibliography  Save this article

Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation

Author

Listed:
  • Rafael E. Carazo-Salas
  • Giulia Guarguaglini

    (CNR, Centre of Evolutionary Genetics, University "La Sapienza")

  • Oliver J. Gruss

    (EMBL)

  • Alexandra Segref

    (EMBL)

  • Eric Karsenti

    (EMBL)

  • Iain W. Mattaj

    (EMBL)

Abstract

Chromosomes are segregated by two antiparallel arrays of microtubules arranged to form the spindle apparatus. During cell division, the nucleation of cytosolic microtubules is prevented and spindle microtubules nucleate from centrosomes (in mitotic animal cells) or around chromosomes (in plants and some meiotic cells)1,2. The molecular mechanism by which chromosomes induce local microtubule nucleation in the absence of centrosomes is unknown3,4,5, but it can be studied by adding chromatin beads to Xenopus egg extracts6. The beads nucleate microtubules that eventually reorganize into a bipolar spindle. RCC1, the guanine-nucleotide-exchange factor for the GTPase protein Ran, is a component of chromatin. Using the chromatin bead assay, we show here that the activity of chromosome-associated RCC1 protein is required for spindle formation. Ran itself, when in the GTP-bound state (Ran-GTP), induces microtubule nucleation and spindle-like structures in M-phase extract. We propose thatRCC1 generates a high local concentration of Ran-GTP around chromatin which in turn induces the local nucleation of microtubules.

Suggested Citation

  • Rafael E. Carazo-Salas & Giulia Guarguaglini & Oliver J. Gruss & Alexandra Segref & Eric Karsenti & Iain W. Mattaj, 1999. "Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation," Nature, Nature, vol. 400(6740), pages 178-181, July.
    • Handle: RePEc:nat:nature:v:400:y:1999:i:6740:d:10.1038_22133
    DOI: 10.1038/22133
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/22133
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/22133?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Bernardo Gouveia & Sagar U. Setru & Matthew R. King & Aaron Hamlin & Howard A. Stone & Joshua W. Shaevitz & Sabine Petry, 2023. "Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Ai Kiyomitsu & Toshiya Nishimura & Shiang Jyi Hwang & Satoshi Ansai & Masato T. Kanemaki & Minoru Tanaka & Tomomi Kiyomitsu, 2024. "Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. Hugo Girão & Joana Macário-Monteiro & Ana C. Figueiredo & Ricardo Silva e Sousa & Elena Doria & Vladimir Demidov & Hugo Osório & Ariana Jacome & Patrick Meraldi & Ekaterina L. Grishchuk & Helder Maiat, 2024. "α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:400:y:1999:i:6740:d:10.1038_22133. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.