Author
Listed:
- Qijun Chen
(Microbiology and Tumor Biology Center, Karolinska Institutet and the Swedish Institute for Infectious Disease Control
Changchun University of Agriculture and Animal Sciences)
- Victor Fernandez
(Microbiology and Tumor Biology Center, Karolinska Institutet and the Swedish Institute for Infectious Disease Control)
- Annika Sundström
(Microbiology and Tumor Biology Center, Karolinska Institutet and the Swedish Institute for Infectious Disease Control)
- Martha Schlichtherle
(Microbiology and Tumor Biology Center, Karolinska Institutet and the Swedish Institute for Infectious Disease Control)
- Santanu Datta
(Astra Research Centre)
- Per Hagblom
(Microbiology and Tumor Biology Center, Karolinska Institutet and the Swedish Institute for Infectious Disease Control)
- Mats Wahlgren
(Microbiology and Tumor Biology Center, Karolinska Institutet and the Swedish Institute for Infectious Disease Control)
Abstract
The protozoan Plasmodium falciparum causes lethal malaria1. Adhesion of erythrocytes infected with P. falciparum to vascular endothelium and to uninfected red blood cells (rosetting) may be involved in the pathogenesis of severe malaria2,3,4. The binding is mediated by the antigenically variant erythrocyte-membrane-protein-1 (PfEMP-1)5,6,7,8, which is encoded by members of the P. falciparum var gene family9,10. The control of expression and switching of var genes seems to lack resemblance to mechanisms operating in variant gene families of other microbial pathogens11,12. Here we show that multiple, distinct var gene transcripts (about 24 or more) can be detected by reverse transcription and polymerase chain reaction in bulk cultures of the rosetting parasite FCR3S1.2, despite the adhesive homogeneity of the cultures. We also detected several var transcripts in single erythrocytes infected with a ring-stage parasite of FCR3S1.2, and found that different var genes are transcribed simultaneously from several chromosomes in the same cell. In contrast, we detected only one var transcript, FCR3S1.2 var-1, which encodes the rosetting PfEMP-1 protein13, in individual rosette-adhesive trophozoite-infected cells, and we found only one PfEMP-1 type at the erythrocyte surface by labelling with 125iodine and immunoprecipitation. We conclude that a single P. falciparum parasite simultaneously transcribes multiple var genes but, through a developmentally regulated process, selects only one PfEMP-1 to reach the surface of the host cell.
Suggested Citation
Qijun Chen & Victor Fernandez & Annika Sundström & Martha Schlichtherle & Santanu Datta & Per Hagblom & Mats Wahlgren, 1998.
"Developmental selection of var gene expression in Plasmodium falciparum,"
Nature, Nature, vol. 394(6691), pages 392-395, July.
Handle:
RePEc:nat:nature:v:394:y:1998:i:6691:d:10.1038_28660
DOI: 10.1038/28660
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Cited by:
- Jaishree Tripathi & Lei Zhu & Sourav Nayak & Michal Stoklasa & Zbynek Bozdech, 2022.
"Stochastic expression of invasion genes in Plasmodium falciparum schizonts,"
Nature Communications, Nature, vol. 13(1), pages 1-15, December.
- Qilong Li & Kunying Lv & Ning Jiang & Tong Liu & Nan Hou & Liying Yu & Yixin Yang & Anni Feng & Yiwei Zhang & Ziwei Su & Xiaoyu Sang & Ying Feng & Ran Chen & Wenyue Xu & Liwang Cui & Yaming Cao & Qiju, 2024.
"SOD3 suppresses early cellular immune responses to parasite infection,"
Nature Communications, Nature, vol. 15(1), pages 1-10, December.
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