IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v394y1998i6689d10.1038_28190.html
   My bibliography  Save this article

Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins

Author

Listed:
  • Gero Miesenböck

    (Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center)

  • Dino A. De Angelis

    (Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center)

  • James E. Rothman

    (Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center)

Abstract

In neural systems, information is often carried by ensembles of cells rather than by individual units. Optical indicators1 provide a powerful means to reveal such distributed activity, particularly when protein-based and encodable in DNA2,3,4: encodable probes can be introduced into cells, tissues, or transgenic organisms by genetic manipulation, selectively expressed in anatomically or functionally defined groups of cells, and, ideally, recorded in situ, without a requirement for exogenous cofactors. Here we describe sensors for secretion and neurotransmission that fulfil these criteria. We have developed pH-sensitive mutants of green fluorescent protein (‘pHluorins’) by structure-directed combinatorial mutagenesis, with the aim of exploiting the acidic pH inside secretory vesicles5,6 to monitor vesicle exocytosis and recycling. When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore ‘flicker’ of single secretory granules.

Suggested Citation

  • Gero Miesenböck & Dino A. De Angelis & James E. Rothman, 1998. "Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins," Nature, Nature, vol. 394(6689), pages 192-195, July.
    • Handle: RePEc:nat:nature:v:394:y:1998:i:6689:d:10.1038_28190
    DOI: 10.1038/28190
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/BF28190
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/28190?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Mable Lam & Koji Takeo & Rafael G. Almeida & Madeline H. Cooper & Kathryn Wu & Manasi Iyer & Husniye Kantarci & J. Bradley Zuchero, 2022. "CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    2. Marley D Kass & Andrew H Moberly & John P McGann, 2013. "Spatiotemporal Alterations in Primary Odorant Representations in Olfactory Marker Protein Knockout Mice," PLOS ONE, Public Library of Science, vol. 8(4), pages 1-10, April.
    3. Joshua J. Rennick & Cameron J. Nowell & Colin W. Pouton & Angus P. R. Johnston, 2022. "Resolving subcellular pH with a quantitative fluorescent lifetime biosensor," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Albert Pineda Rodó & Libuše Váchová & Zdena Palková, 2012. "In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach," PLOS ONE, Public Library of Science, vol. 7(3), pages 1-12, March.
    5. Shanley F. Longfield & Mahdie Mollazade & Tristan P. Wallis & Rachel S. Gormal & Merja Joensuu & Jesse R. Wark & Ashley J. Waardenberg & Christopher Small & Mark E. Graham & Frédéric A. Meunier & Ramó, 2023. "Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    6. Jung-Hwan Choi & Lauren Bayer Horowitz & Niels Ringstad, 2021. "Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    7. Christian Hoffmann & Jakob Rentsch & Taka A. Tsunoyama & Akshita Chhabra & Gerard Aguilar Perez & Rajdeep Chowdhury & Franziska Trnka & Aleksandr A. Korobeinikov & Ali H. Shaib & Marcelo Ganzella & Gr, 2023. "Synapsin condensation controls synaptic vesicle sequestering and dynamics," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:394:y:1998:i:6689:d:10.1038_28190. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.