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High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules

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  • George R. Heath

    (Weill Cornell Medicine, Department of Anesthesiology
    Weill Cornell Medicine, Department of Physiology and Biophysics)

  • Simon Scheuring

    (Weill Cornell Medicine, Department of Anesthesiology
    Weill Cornell Medicine, Department of Physiology and Biophysics)

Abstract

Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally.

Suggested Citation

  • George R. Heath & Simon Scheuring, 2018. "High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07512-3
    DOI: 10.1038/s41467-018-07512-3
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    Cited by:

    1. Alma P. Perrino & Atsushi Miyagi & Simon Scheuring, 2021. "Single molecule kinetics of bacteriorhodopsin by HS-AFM," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    2. Yining Jiang & Batiste Thienpont & Vinay Sapuru & Richard K. Hite & Jeremy S. Dittman & James N. Sturgis & Simon Scheuring, 2022. "Membrane-mediated protein interactions drive membrane protein organization," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Yangang Pan & Jingyu Zhan & Yining Jiang & Di Xia & Simon Scheuring, 2023. "A concerted ATPase cycle of the protein transporter AAA-ATPase Bcs1," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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