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Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells

Author

Listed:
  • Joseph Jose Thottacherry

    (Tata Institute of Fundamental Research (TIFR))

  • Anita Joanna Kosmalska

    (Institute for Bioengineering of Catalonia (IBEC)
    University of Barcelona)

  • Amit Kumar

    (Raman Research Institute)

  • Amit Singh Vishen

    (Tata Institute of Fundamental Research (TIFR)
    National Centre for Biological Sciences (NCBS))

  • Alberto Elosegui-Artola

    (Institute for Bioengineering of Catalonia (IBEC))

  • Susav Pradhan

    (Raman Research Institute)

  • Sumit Sharma

    (CSIR - Indian Institute of Integrative Medicine)

  • Parvinder P. Singh

    (CSIR - Indian Institute of Integrative Medicine)

  • Marta C. Guadamillas

    (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))

  • Natasha Chaudhary

    (Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis
    Weill Cornell Medical College)

  • Ram Vishwakarma

    (CSIR - Indian Institute of Integrative Medicine)

  • Xavier Trepat

    (Institute for Bioengineering of Catalonia (IBEC)
    University of Barcelona
    Biomateriales y Nanomedicina (CIBER-BBN) and Institució Catalana de Recerca i Estudis Avançats (ICREA))

  • Miguel A. Pozo

    (Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC))

  • Robert G. Parton

    (Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis)

  • Madan Rao

    (Tata Institute of Fundamental Research (TIFR)
    National Centre for Biological Sciences (NCBS))

  • Pramod Pullarkat

    (Raman Research Institute)

  • Pere Roca-Cusachs

    (Institute for Bioengineering of Catalonia (IBEC)
    University of Barcelona)

  • Satyajit Mayor

    (Tata Institute of Fundamental Research (TIFR)
    Tata Institute of Fundamental Research (TIFR))

Abstract

Plasma membrane tension regulates many key cellular processes. It is modulated by, and can modulate, membrane trafficking. However, the cellular pathway(s) involved in this interplay is poorly understood. Here we find that, among a number of endocytic processes operating simultaneously at the cell surface, a dynamin independent pathway, the CLIC/GEEC (CG) pathway, is rapidly and specifically upregulated upon a sudden reduction of tension. Moreover, inhibition (activation) of the CG pathway results in lower (higher) membrane tension. However, alteration in membrane tension does not directly modulate CG endocytosis. This requires vinculin, a mechano-transducer recruited to focal adhesion in adherent cells. Vinculin acts by controlling the levels of a key regulator of the CG pathway, GBF1, at the plasma membrane. Thus, the CG pathway directly regulates membrane tension and is in turn controlled via a mechano-chemical feedback inhibition, potentially leading to homeostatic regulation of membrane tension in adherent cells.

Suggested Citation

  • Joseph Jose Thottacherry & Anita Joanna Kosmalska & Amit Kumar & Amit Singh Vishen & Alberto Elosegui-Artola & Susav Pradhan & Sumit Sharma & Parvinder P. Singh & Marta C. Guadamillas & Natasha Chaudh, 2018. "Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06738-5
    DOI: 10.1038/s41467-018-06738-5
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    Cited by:

    1. Derin Sevenler & Mehmet Toner, 2024. "High throughput intracellular delivery by viscoelastic mechanoporation," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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