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Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga

Author

Listed:
  • Yuqun Xu

    (The University of Tokyo)

  • Takuya Miyakawa

    (The University of Tokyo)

  • Shohei Nosaki

    (The University of Tokyo)

  • Akira Nakamura

    (The University of Tokyo)

  • Ying Lyu

    (The University of Tokyo)

  • Hidemitsu Nakamura

    (The University of Tokyo)

  • Umeharu Ohto

    (The University of Tokyo)

  • Hanako Ishida

    (The University of Tokyo)

  • Toshiyuki Shimizu

    (The University of Tokyo)

  • Tadao Asami

    (The University of Tokyo
    King Abdulaziz University)

  • Masaru Tanokura

    (The University of Tokyo)

Abstract

HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we show that Striga hermonthica HTL proteins (ShHTLs) in ‘conserved’ and ‘intermediate’ clades are able to bind karrikin. The ‘divergent’ clade is able to hydrolyze strigolactone. Unexpectedly, we find that ShD14 is also capable of hydrolyzing strigolactone. Through comparative analysis of ShHTLs and ShD14 crystal structures, we provide insights into the basis for their selectivity. Moreover, we show that both ShD14 and divergent clade ShHTLs, but not conserved and intermediate clade ShHTLs, can interact with the putative downstream signaling component ShMAX2 in the presence of the synthetic strigolactone, rac-GR24. These findings provide insight into how strigolactone is perceived and how ligand specificity is determined.

Suggested Citation

  • Yuqun Xu & Takuya Miyakawa & Shohei Nosaki & Akira Nakamura & Ying Lyu & Hidemitsu Nakamura & Umeharu Ohto & Hanako Ishida & Toshiyuki Shimizu & Tadao Asami & Masaru Tanokura, 2018. "Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06452-2
    DOI: 10.1038/s41467-018-06452-2
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    Cited by:

    1. Dawei Wang & Zhili Pang & Haiyang Yu & Benjamin Thiombiano & Aimee Walmsley & Shuyi Yu & Yingying Zhang & Tao Wei & Lu Liang & Jing Wang & Xin Wen & Harro J. Bouwmeester & Ruifeng Yao & Zhen Xi, 2022. "Probing strigolactone perception mechanisms with rationally designed small-molecule agonists stimulating germination of root parasitic weeds," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Satoshi Ogawa & Songkui Cui & Alexandra R. F. White & David C. Nelson & Satoko Yoshida & Ken Shirasu, 2022. "Strigolactones are chemoattractants for host tropism in Orobanchaceae parasitic plants," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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