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Mapping of histone-binding sites in histone replacement-completed spermatozoa

Author

Listed:
  • Keisuke Yoshida

    (Cluster for Pioneering Research, CREST Research Project of JST (Japan Science and Technology Agency), RIKEN Tsukuba Institute)

  • Masafumi Muratani

    (University of Tsukuba)

  • Hiromitsu Araki

    (Kyushu University Graduate School of Medical Sciences)

  • Fumihito Miura

    (Kyushu University Graduate School of Medical Sciences)

  • Takehiro Suzuki

    (RIKEN Center for Sustainable Resource Science)

  • Naoshi Dohmae

    (RIKEN Center for Sustainable Resource Science)

  • Yuki Katou

    (The University of Tokyo)

  • Katsuhiko Shirahige

    (The University of Tokyo)

  • Takashi Ito

    (Kyushu University Graduate School of Medical Sciences)

  • Shunsuke Ishii

    (Cluster for Pioneering Research, CREST Research Project of JST (Japan Science and Technology Agency), RIKEN Tsukuba Institute
    University of Tsukuba)

Abstract

The majority of histones are replaced by protamines during spermatogenesis, but small amounts are retained in mammalian spermatozoa. Since nucleosomes in spermatozoa influence epigenetic inheritance, it is important to know how histones are distributed in the sperm genome. Conflicting data, which may result from different conditions used for micrococcal nuclease (MNase) digestion, have been reported: retention of nucleosomes at either gene promoter regions or within distal gene-poor regions. Here, we find that the swim-up sperm used in many studies contain about 10% population of sperm which have not yet completed the histone-to-protamine replacement. We develop a method to purify histone replacement-completed sperm (HRCS) and to completely solubilize histones from cross-linked HRCS without MNase digestion. Our results indicate that histones are retained at specific promoter regions in HRCS. This method allows the study of epigenetic status in mature sperm.

Suggested Citation

  • Keisuke Yoshida & Masafumi Muratani & Hiromitsu Araki & Fumihito Miura & Takehiro Suzuki & Naoshi Dohmae & Yuki Katou & Katsuhiko Shirahige & Takashi Ito & Shunsuke Ishii, 2018. "Mapping of histone-binding sites in histone replacement-completed spermatozoa," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06243-9
    DOI: 10.1038/s41467-018-06243-9
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    Cited by:

    1. Grigorios Fanourgakis & Laura Gaspa-Toneu & Pavel A. Komarov & Panagiotis Papasaikas & Evgeniy A. Ozonov & Sebastien A. Smallwood & Antoine H. F. M. Peters, 2025. "DNA methylation modulates nucleosome retention in sperm and H3K4 methylation deposition in early mouse embryos," Nature Communications, Nature, vol. 16(1), pages 1-22, December.

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