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A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein

Author

Listed:
  • Anzhong Li

    (The Ohio State University)

  • Jingyou Yu

    (The Ohio State University
    The Ohio State University)

  • Mijia Lu

    (The Ohio State University)

  • Yuanmei Ma

    (The Ohio State University)

  • Zayed Attia

    (The Ohio State University)

  • Chao Shan

    (University of Texas Medical Branch)

  • Miaoge Xue

    (The Ohio State University)

  • Xueya Liang

    (The Ohio State University)

  • Kelsey Craig

    (The Ohio State University)

  • Nirajkumar Makadiya

    (The Ohio State University)

  • Jennifer J. He

    (The Ohio State University)

  • Ryan Jennings

    (The Ohio State University)

  • Pei-Yong Shi

    (University of Texas Medical Branch)

  • Mark E. Peeples

    (The Research Institute at Nationwide Children’s Hospital
    The Ohio State University)

  • Shan-Lu Liu

    (The Ohio State University
    The Ohio State University
    The Ohio State University
    The Ohio State University)

  • Prosper N. Boyaka

    (The Ohio State University
    The Ohio State University)

  • Jianrong Li

    (The Ohio State University
    The Ohio State University)

Abstract

Current efforts to develop Zika virus (ZIKV) subunit vaccines have been focused on pre-membrane (prM) and envelope (E) proteins, but the role of NS1 in ZIKV-specific immune response and protection is poorly understood. Here, we develop an attenuated recombinant vesicular stomatitis virus (rVSV)-based vaccine expressing ZIKV prM-E-NS1 as a polyprotein. This vectored vaccine candidate is attenuated in mice, where a single immunization induces ZIKV-specific antibody and T cell immune responses that provide protection against ZIKV challenge. Co-expression of prM, E, and NS1 induces significantly higher levels of Th2 and Th17 cytokine responses than prM-E. In addition, NS1 alone is capable of conferring partial protection against ZIKV infection in mice even though it does not induce neutralizing antibodies. These results demonstrate that attenuated rVSV co-expressing prM, E, and NS1 is a promising vaccine candidate for protection against ZIKV infection and highlights an important role for NS1 in ZIKV-specific cellular immune responses.

Suggested Citation

  • Anzhong Li & Jingyou Yu & Mijia Lu & Yuanmei Ma & Zayed Attia & Chao Shan & Miaoge Xue & Xueya Liang & Kelsey Craig & Nirajkumar Makadiya & Jennifer J. He & Ryan Jennings & Pei-Yong Shi & Mark E. Peep, 2018. "A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05276-4
    DOI: 10.1038/s41467-018-05276-4
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    Cited by:

    1. Yuexiu Zhang & Michelle Chamblee & Jiayu Xu & Panke Qu & Mohamed M. Shamseldin & Sung J. Yoo & Jack Misny & Ilada Thongpan & Mahesh KC & Jesse M. Hall & Yash A. Gupta & John P. Evans & Mijia Lu & Chen, 2024. "Three SARS-CoV-2 spike protein variants delivered intranasally by measles and mumps vaccines are broadly protective," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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