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Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development

Author

Listed:
  • Heather J. Painter

    (The Pennsylvania State University
    The Pennsylvania State University)

  • Neo Christopher Chung

    (Princeton University
    University of Warsaw)

  • Aswathy Sebastian

    (The Pennsylvania State University)

  • Istvan Albert

    (The Pennsylvania State University)

  • John D. Storey

    (Princeton University
    Princeton University)

  • Manuel Llinás

    (The Pennsylvania State University
    The Pennsylvania State University
    The Pennsylvania State University)

Abstract

Genome-wide analysis of transcription in the malaria parasite Plasmodium falciparum has revealed robust variation in steady-state mRNA abundance throughout the 48-h intraerythrocytic developmental cycle (IDC), suggesting that this process is highly dynamic and tightly regulated. Here, we utilize rapid 4-thiouracil (4-TU) incorporation via pyrimidine salvage to specifically label, capture, and quantify newly-synthesized RNA transcripts at every hour throughout the IDC. This high-resolution global analysis of the transcriptome captures the timing and rate of transcription for each newly synthesized mRNA in vivo, revealing active transcription throughout all IDC stages. Using a statistical model to predict the mRNA dynamics contributing to the total mRNA abundance at each timepoint, we find varying degrees of transcription and stabilization for each mRNA corresponding to developmental transitions. Finally, our results provide new insight into co-regulation of mRNAs throughout the IDC through regulatory DNA sequence motifs, thereby expanding our understanding of P. falciparum mRNA dynamics.

Suggested Citation

  • Heather J. Painter & Neo Christopher Chung & Aswathy Sebastian & Istvan Albert & John D. Storey & Manuel Llinás, 2018. "Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04966-3
    DOI: 10.1038/s41467-018-04966-3
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    Cited by:

    1. Mwikali Kioko & Alena Pance & Shaban Mwangi & David Goulding & Alison Kemp & Martin Rono & Lynette Isabella Ochola-Oyier & Pete C. Bull & Philip Bejon & Julian C. Rayner & Abdirahman I. Abdi, 2023. "Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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