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Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks

Author

Listed:
  • Teppei Ebina

    (The University of Tokyo
    National Institute for Basic Biology)

  • Yoshito Masamizu

    (The University of Tokyo
    National Institute for Basic Biology
    SOKENDAI (The Graduate University for Advanced Studies))

  • Yasuhiro R. Tanaka

    (The University of Tokyo
    National Institute for Basic Biology)

  • Akiya Watakabe

    (RIKEN Center for Brain Science)

  • Reiko Hirakawa

    (National Institute for Basic Biology
    Central Institute for Experimental Animals)

  • Yuka Hirayama

    (The University of Tokyo)

  • Riichiro Hira

    (National Institute for Basic Biology
    SOKENDAI (The Graduate University for Advanced Studies))

  • Shin-Ichiro Terada

    (The University of Tokyo
    National Institute for Basic Biology)

  • Daisuke Koketsu

    (National Institute for Physiological Sciences)

  • Kazuo Hikosaka

    (Kawasaki University of Medical Welfare)

  • Hiroaki Mizukami

    (Jichi Medical University)

  • Atsushi Nambu

    (SOKENDAI (The Graduate University for Advanced Studies)
    National Institute for Physiological Sciences)

  • Erika Sasaki

    (Central Institute for Experimental Animals
    Keio University)

  • Tetsuo Yamamori

    (RIKEN Center for Brain Science)

  • Masanori Matsuzaki

    (The University of Tokyo
    National Institute for Basic Biology
    SOKENDAI (The Graduate University for Advanced Studies)
    The University Tokyo Institutes for Advanced Study)

Abstract

Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution. However, marmosets have posed a challenge due to limited success in training on motor tasks. Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. After 2–5 months of training sessions, head-fixed marmosets can control a manipulandum to move a cursor to a target on a screen. We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions. In a two-target reaching task, some neurons show direction-selective activity over the training days. In a short-term force-field adaptation task, some neurons change their activity when the force field is on. Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition.

Suggested Citation

  • Teppei Ebina & Yoshito Masamizu & Yasuhiro R. Tanaka & Akiya Watakabe & Reiko Hirakawa & Yuka Hirayama & Riichiro Hira & Shin-Ichiro Terada & Daisuke Koketsu & Kazuo Hikosaka & Hiroaki Mizukami & Atsu, 2018. "Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04286-6
    DOI: 10.1038/s41467-018-04286-6
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    Cited by:

    1. Shinnosuke Nomura & Shin-Ichiro Terada & Teppei Ebina & Masato Uemura & Yoshito Masamizu & Kenichi Ohki & Masanori Matsuzaki, 2024. "ARViS: a bleed-free multi-site automated injection robot for accurate, fast, and dense delivery of virus to mouse and marmoset cerebral cortex," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    2. Teppei Ebina & Akitaka Sasagawa & Dokyeong Hong & Rieko Setsuie & Keitaro Obara & Yoshito Masamizu & Masashi Kondo & Shin-Ichiro Terada & Katsuya Ozawa & Masato Uemura & Masafumi Takaji & Akiya Wataka, 2024. "Dynamics of directional motor tuning in the primate premotor and primary motor cortices during sensorimotor learning," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    3. Keitaro Obara & Teppei Ebina & Shin-Ichiro Terada & Takanori Uka & Misako Komatsu & Masafumi Takaji & Akiya Watakabe & Kenta Kobayashi & Yoshito Masamizu & Hiroaki Mizukami & Tetsuo Yamamori & Kiyoto , 2023. "Change detection in the primate auditory cortex through feedback of prediction error signals," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    4. Jimin Wu & Yuzhi Chen & Ashok Veeraraghavan & Eyal Seidemann & Jacob T. Robinson, 2024. "Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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