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Structural basis of actin monomer re-charging by cyclase-associated protein

Author

Listed:
  • Tommi Kotila

    (University of Helsinki)

  • Konstantin Kogan

    (University of Helsinki)

  • Giray Enkavi

    (University of Helsinki)

  • Siyang Guo

    (Brandeis University)

  • Ilpo Vattulainen

    (University of Helsinki
    Tampere University of Technology)

  • Bruce L. Goode

    (Brandeis University)

  • Pekka Lappalainen

    (University of Helsinki)

Abstract

Actin polymerization powers key cellular processes, including motility, morphogenesis, and endocytosis. The actin turnover cycle depends critically on “re-charging” of ADP-actin monomers with ATP, but whether this reaction requires dedicated proteins in cells, and the underlying mechanism, have remained elusive. Here we report that nucleotide exchange catalyzed by the ubiquitous cytoskeletal regulator cyclase-associated protein (CAP) is critical for actin-based processes in vivo. We determine the structure of the CAP–actin complex, which reveals that nucleotide exchange occurs in a compact, sandwich-like complex formed between the dimeric actin-binding domain of CAP and two ADP-actin monomers. In the crystal structure, the C-terminal tail of CAP associates with the nucleotide-sensing region of actin, and this interaction is required for rapid re-charging of actin by both yeast and mammalian CAPs. These data uncover the conserved structural basis and biological role of protein-catalyzed re-charging of actin monomers.

Suggested Citation

  • Tommi Kotila & Konstantin Kogan & Giray Enkavi & Siyang Guo & Ilpo Vattulainen & Bruce L. Goode & Pekka Lappalainen, 2018. "Structural basis of actin monomer re-charging by cyclase-associated protein," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04231-7
    DOI: 10.1038/s41467-018-04231-7
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    Cited by:

    1. Tommi Kotila & Hugo Wioland & Muniyandi Selvaraj & Konstantin Kogan & Lina Antenucci & Antoine Jégou & Juha T. Huiskonen & Guillaume Romet-Lemonne & Pekka Lappalainen, 2022. "Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Micaela Boiero Sanders & Wout Oosterheert & Oliver Hofnagel & Peter Bieling & Stefan Raunser, 2024. "Phalloidin and DNase I-bound F-actin pointed end structures reveal principles of filament stabilization and disassembly," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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