IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-04161-4.html
   My bibliography  Save this article

Supramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging

Author

Listed:
  • Kyung Lock Kim

    (Institute for Basic Science (IBS))

  • Gihyun Sung

    (Pohang University of Science and Technology (POSTECH))

  • Jaehwan Sim

    (Pohang University of Science and Technology (POSTECH))

  • James Murray

    (Institute for Basic Science (IBS))

  • Meng Li

    (Institute for Basic Science (IBS))

  • Ara Lee

    (Pohang University of Science and Technology (POSTECH))

  • Annadka Shrinidhi

    (Institute for Basic Science (IBS))

  • Kyeng Min Park

    (Institute for Basic Science (IBS)
    University of Science and Technology (UST))

  • Kimoon Kim

    (Institute for Basic Science (IBS)
    Pohang University of Science and Technology (POSTECH)
    Pohang University of Science and Technology (POSTECH)
    Pohang University of Science and Technology (POSTECH))

Abstract

Here we report ultrastable synthetic binding pairs between cucurbit[7]uril (CB[7]) and adamantyl- (AdA) or ferrocenyl-ammonium (FcA) as a supramolecular latching system for protein imaging, overcoming the limitations of protein-based binding pairs. Cyanine 3-conjugated CB[7] (Cy3-CB[7]) can visualize AdA- or FcA-labeled proteins to provide clear fluorescence images for accurate and precise analysis of proteins. Furthermore, controllability of the system is demonstrated by treating with a stronger competitor guest. At low temperature, this allows us to selectively detach Cy3-CB[7] from guest-labeled proteins on the cell surface, while leaving Cy3-CB[7] latched to the cytosolic proteins for spatially conditional visualization of target proteins. This work represents a non-protein-based bioimaging tool which has inherent advantages over the widely used protein-based techniques, thereby demonstrating the great potential of this synthetic system.

Suggested Citation

  • Kyung Lock Kim & Gihyun Sung & Jaehwan Sim & James Murray & Meng Li & Ara Lee & Annadka Shrinidhi & Kyeng Min Park & Kimoon Kim, 2018. "Supramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04161-4
    DOI: 10.1038/s41467-018-04161-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-04161-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-04161-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yinglong Wu & Lihe Sun & Xiaokai Chen & Jiawei Liu & Juan Ouyang & Xiaodong Zhang & Yi Guo & Yun Chen & Wei Yuan & Dongdong Wang & Ting He & Fang Zeng & Hongzhong Chen & Shuizhu Wu & Yanli Zhao, 2023. "Cucurbit[8]uril-based water-dispersible assemblies with enhanced optoacoustic performance for multispectral optoacoustic imaging," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Ara Lee & Gihyun Sung & Sanghee Shin & Song-Yi Lee & Jaehwan Sim & Truong Thi My Nhung & Tran Diem Nghi & Sang Ki Park & Ponnusamy Pon Sathieshkumar & Imkyeung Kang & Ji Young Mun & Jong-Seo Kim & Hyu, 2024. "OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04161-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.