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De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis

Author

Listed:
  • Chunming Ding

    (Wenzhou Medical University)

  • Yen Ching Lim

    (Wenzhou Medical University
    Duke-NUS Medical School)

  • Sook Yoong Chia

    (Duke-NUS Medical School)

  • Arcinas Camille Esther Walet

    (Duke-NUS Medical School)

  • Shaohai Xu

    (Nanyang Technological University)

  • Kinyui Alice Lo

    (Institute of Molecular and Cell Biology)

  • Yanling Zhao

    (Wenzhou Medical University)

  • Dewen Zhu

    (Wenzhou Medical University)

  • Zhihui Shan

    (Wenzhou Medical University)

  • Qingfeng Chen

    (Institute of Molecular and Cell Biology)

  • Melvin Khee-Shing Leow

    (Duke-NUS Medical School
    Agency for Science, Technology and Research (A*STAR) and National University Health System (NUHS)
    National University Health System (NUHS)
    Tan Tock Seng Hospital)

  • Dan Xu

    (Wenzhou Medical University
    Duke-NUS Medical School)

  • Lei Sun

    (Duke-NUS Medical School
    Institute of Molecular and Cell Biology)

Abstract

Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.

Suggested Citation

  • Chunming Ding & Yen Ching Lim & Sook Yoong Chia & Arcinas Camille Esther Walet & Shaohai Xu & Kinyui Alice Lo & Yanling Zhao & Dewen Zhu & Zhihui Shan & Qingfeng Chen & Melvin Khee-Shing Leow & Dan Xu, 2018. "De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03754-3
    DOI: 10.1038/s41467-018-03754-3
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    Cited by:

    1. Yan-Ting Chen & Qi-Yuan Yang & Yun Hu & Xiang-Dong Liu & Jeanene M. Avila & Mei-Jun Zhu & Peter W. Nathanielsz & Min Du, 2021. "Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance," Nature Communications, Nature, vol. 12(1), pages 1-18, December.

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