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Metal-organic frameworks for precise inclusion of single-stranded DNA and transfection in immune cells

Author

Listed:
  • Shuang Peng

    (Wuhan University)

  • Binglin Bie

    (Wuhan University
    UC Berkeley-Wuhan University Joint Innovative Center, The Institute of Advanced Studies, Wuhan University)

  • Yangzesheng Sun

    (Wuhan University
    UC Berkeley-Wuhan University Joint Innovative Center, The Institute of Advanced Studies, Wuhan University)

  • Min Liu

    (Wuhan University)

  • Hengjiang Cong

    (Wuhan University)

  • Wentao Zhou

    (Wuhan University)

  • Yucong Xia

    (Wuhan University)

  • Heng Tang

    (Wuhan University)

  • Hexiang Deng

    (Wuhan University
    UC Berkeley-Wuhan University Joint Innovative Center, The Institute of Advanced Studies, Wuhan University)

  • Xiang Zhou

    (Wuhan University
    UC Berkeley-Wuhan University Joint Innovative Center, The Institute of Advanced Studies, Wuhan University)

Abstract

Effective transfection of genetic molecules such as DNA usually relies on vectors that can reversibly uptake and release these molecules, and protect them from digestion by nuclease. Non-viral vectors meeting these requirements are rare due to the lack of specific interactions with DNA. Here, we design a series of four isoreticular metal-organic frameworks (Ni-IRMOF-74-II to -V) with progressively tuned pore size from 2.2 to 4.2 nm to precisely include single-stranded DNA (ssDNA, 11–53 nt), and to achieve reversible interaction between MOFs and ssDNA. The entire nucleic acid chain is completely confined inside the pores providing excellent protection, and the geometric distribution of the confined ssDNA is visualized by X-ray diffraction. Two MOFs in this series exhibit excellent transfection efficiency in mammalian immune cells, 92% in the primary mouse immune cells (CD4+ T cell) and 30% in human immune cells (THP-1 cell), unrivaled by the commercialized agents (Lipo and Neofect).

Suggested Citation

  • Shuang Peng & Binglin Bie & Yangzesheng Sun & Min Liu & Hengjiang Cong & Wentao Zhou & Yucong Xia & Heng Tang & Hexiang Deng & Xiang Zhou, 2018. "Metal-organic frameworks for precise inclusion of single-stranded DNA and transfection in immune cells," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03650-w
    DOI: 10.1038/s41467-018-03650-w
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    Cited by:

    1. Xiaoli Tian & Fu Li & Zhenyuan Tang & Song Wang & Kangkang Weng & Dan Liu & Shaoyong Lu & Wangyu Liu & Zhong Fu & Wenjun Li & Hengwei Qiu & Min Tu & Hao Zhang & Jinghong Li, 2024. "Crosslinking-induced patterning of MOFs by direct photo- and electron-beam lithography," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Danping Tian & Ruipeng Hao & Xiaoming Zhang & Hu Shi & Yuwei Wang & Linfeng Liang & Haichao Liu & Hengquan Yang, 2023. "Multi-compartmental MOF microreactors derived from Pickering double emulsions for chemo-enzymatic cascade catalysis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Tomoya Iizuka & Hiroyuki Sano & Benjamin Ouay & Nobuhiko Hosono & Takashi Uemura, 2023. "An approach to MOFaxanes by threading ultralong polymers through metal–organic framework microcrystals," Nature Communications, Nature, vol. 14(1), pages 1-8, December.

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