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TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice

Author

Listed:
  • Deqiang Ding

    (Michigan State University)

  • Jiali Liu

    (Michigan State University
    China Agricultural University)

  • Uros Midic

    (Michigan State University)

  • Yingjie Wu

    (Michigan State University
    China Agricultural University)

  • Kunzhe Dong

    (Avian Disease and Oncology Laboratory)

  • Ashley Melnick

    (Michigan State University)

  • Keith E. Latham

    (Michigan State University
    Michigan State University
    Michigan State University)

  • Chen Chen

    (Michigan State University
    Michigan State University
    Michigan State University)

Abstract

Pachytene piRNAs are the most abundant piRNAs in mammalian adult testes. They are generated from long precursor transcripts by the primary piRNA biogenesis pathway but the factors involved in pachytene piRNA precursors processing are poorly understood. Here we show that the Tudor domain-containing 5 (TDRD5) protein is essential for pachytene piRNA biogenesis in mice. Conditional inactivation of TDRD5 in mouse postnatal germ cells reveals that TDRD5 selectively regulates the production of pachytene piRNAs from abundant piRNA-producing precursors, with little effect on low-abundant piRNAs. Unexpectedly, TDRD5 is not required for the 5′ end processing of the precursors, but is crucial for promoting production of piRNAs from the other regions of the transcript. Furthermore, we show that TDRD5 is an RNA-binding protein directly associating with piRNA precursors. These observations establish TDRD5 as a piRNA biogenesis factor and reveal two genetically separable steps at the start of pachytene piRNA processing.

Suggested Citation

  • Deqiang Ding & Jiali Liu & Uros Midic & Yingjie Wu & Kunzhe Dong & Ashley Melnick & Keith E. Latham & Chen Chen, 2018. "TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02622-w
    DOI: 10.1038/s41467-017-02622-w
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    Cited by:

    1. Yu H. Sun & Ruoqiao Huiyi Wang & Khai Du & Jiang Zhu & Jihong Zheng & Li Huitong Xie & Amanda A. Pereira & Chao Zhang & Emiliano P. Ricci & Xin Zhiguo Li, 2021. "Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    2. Huan Wei & Jie Gao & Di-Hang Lin & Ruirong Geng & Jiaoyang Liao & Tian-Yu Huang & Guanyi Shang & Jiongjie Jing & Zong-Wei Fan & Duo Pan & Zi-Qi Yin & Tianming Li & Xinyu Liu & Shuang Zhao & Chen Chen , 2024. "piRNA loading triggers MIWI translocation from the intermitochondrial cement to chromatoid body during mouse spermatogenesis," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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