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In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure

Author

Listed:
  • Sivanandane Sittadjody

    (Wake Forest School for Medicine)

  • Justin M. Saul

    (Paper and Biomedical Engineering, Miami University)

  • John P. McQuilling

    (Wake Forest School for Medicine
    Wake Forest School of Medicine)

  • Sunyoung Joo

    (Wake Forest School for Medicine)

  • Thomas C. Register

    (Wake Forest University School of Medicine)

  • James J. Yoo

    (Wake Forest School for Medicine
    Wake Forest School of Medicine)

  • Anthony Atala

    (Wake Forest School for Medicine
    Wake Forest School of Medicine)

  • Emmanuel C. Opara

    (Wake Forest School for Medicine
    Wake Forest School of Medicine)

Abstract

Safe clinical hormone replacement (HR) will likely become increasingly important in the growing populations of aged women and cancer patients undergoing treatments that ablate the ovaries. Cell-based HRT (cHRT) is an alternative approach that may allow certain physiological outcomes to be achieved with lower circulating hormone levels than pharmacological means due to participation of cells in the hypothalamus-pituitary-ovary feedback control loop. Here we describe the in vivo performance of 3D bioengineered ovarian constructs that recapitulate native cell–cell interactions between ovarian granulosa and theca cells as an approach to cHRT. The constructs are fabricated using either Ca++ or Sr++ to crosslink alginate. Following implantation in ovariectomized (ovx) rats, the Sr++-cross-linked constructs achieve stable secretion of hormones during 90 days of study. Further, we show these constructs with isogeneic cells to be effective in ameliorating adverse effects of hormone deficiency, including bone health, uterine health, and body composition in this rat model.

Suggested Citation

  • Sivanandane Sittadjody & Justin M. Saul & John P. McQuilling & Sunyoung Joo & Thomas C. Register & James J. Yoo & Anthony Atala & Emmanuel C. Opara, 2017. "In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01851-3
    DOI: 10.1038/s41467-017-01851-3
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    Cited by:

    1. Yunhui Liu & Lu Zhang & Nan Hu & Jie Shao & Dazhi Yang & Changshun Ruan & Shishu Huang & Liping Wang & William W. Lu & Xinzhou Zhang & Fan Yang, 2022. "An optogenetic approach for regulating human parathyroid hormone secretion," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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