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In situ detection of the protein corona in complex environments

Author

Listed:
  • Monica Carril

    (CIC biomaGUNE
    Ikerbasque, Basque Foundation for Science)

  • Daniel Padro

    (CIC biomaGUNE)

  • Pablo del Pino

    (CIC biomaGUNE
    Philipps Universität Marburg
    Universidade de Santiago de Compostela)

  • Carolina Carrillo-Carrion

    (CIC biomaGUNE)

  • Marta Gallego

    (CIC biomaGUNE)

  • Wolfgang J. Parak

    (CIC biomaGUNE
    Philipps Universität Marburg
    Universität Hamburg)

Abstract

Colloidal nanoparticles (NPs) are a versatile potential platform for in vivo nanomedicine. Inside blood circulation, NPs may undergo drastic changes, such as by formation of a protein corona. The in vivo corona cannot be completely emulated by the corona formed in blood. Thus, in situ detection in complex media, and ultimately in vivo, is required. Here we present a methodology for determining protein corona formation in complex media. NPs are labeled with 19F and their diffusion coefficient measured using 19F diffusion-ordered nuclear magnetic resonance (NMR) spectroscopy. 19F diffusion NMR measurements of hydrodynamic radii allow for in situ characterization of NPs in complex environments by quantification of protein adsorption to the surface of NPs, as determined by increase in hydrodynamic radius. The methodology is not optics based, and thus can be used in turbid environments, as in the presence of cells.

Suggested Citation

  • Monica Carril & Daniel Padro & Pablo del Pino & Carolina Carrillo-Carrion & Marta Gallego & Wolfgang J. Parak, 2017. "In situ detection of the protein corona in complex environments," Nature Communications, Nature, vol. 8(1), pages 1-5, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01826-4
    DOI: 10.1038/s41467-017-01826-4
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    Cited by:

    1. Huijie Yan & Michele Cacioppo & Saad Megahed & Francesca Arcudi & Luka Đorđević & Dingcheng Zhu & Florian Schulz & Maurizio Prato & Wolfgang J. Parak & Neus Feliu, 2021. "Influence of the chirality of carbon nanodots on their interaction with proteins and cells," Nature Communications, Nature, vol. 12(1), pages 1-14, December.

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