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Gradient light interference microscopy for 3D imaging of unlabeled specimens

Author

Listed:
  • Tan H. Nguyen

    (University of Illinois at Urbana-Champaign)

  • Mikhail E. Kandel

    (University of Illinois at Urbana-Champaign)

  • Marcello Rubessa

    (University of Illinois at Urbana-Champaign)

  • Matthew B. Wheeler

    (University of Illinois at Urbana-Champaign)

  • Gabriel Popescu

    (University of Illinois at Urbana-Champaign)

Abstract

Multiple scattering limits the contrast in optical imaging of thick specimens. Here, we present gradient light interference microscopy (GLIM) to extract three-dimensional information from both thin and thick unlabeled specimens. GLIM exploits a special case of low-coherence interferometry to extract phase information from the specimen, which in turn can be used to measure cell mass, volume, surface area, and their evolutions in time. Because it combines multiple intensity images that correspond to controlled phase shifts between two interfering waves, gradient light interference microscopy is capable of suppressing the incoherent background due to multiple scattering. GLIM can potentially become a valuable tool for in vitro fertilization, where contrast agents and fluorophores may impact the viability of the embryo. Since GLIM is implemented as an add-on module to an existing inverted microscope, we anticipate that it will be adopted rapidly by the biological community.

Suggested Citation

  • Tan H. Nguyen & Mikhail E. Kandel & Marcello Rubessa & Matthew B. Wheeler & Gabriel Popescu, 2017. "Gradient light interference microscopy for 3D imaging of unlabeled specimens," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00190-7
    DOI: 10.1038/s41467-017-00190-7
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    Cited by:

    1. Michelle Küppers & David Albrecht & Anna D. Kashkanova & Jennifer Lühr & Vahid Sandoghdar, 2023. "Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Xinwei Wang & Hao Wang & Jinlu Wang & Xingsi Liu & Huijie Hao & You Sin Tan & Yilei Zhang & He Zhang & Xiangyan Ding & Weisong Zhao & Yuhang Wang & Zhengang Lu & Jian Liu & Joel K. W. Yang & Jiubin Ta, 2023. "Single-shot isotropic differential interference contrast microscopy," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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