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Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system

Author

Listed:
  • Yihan Zhang

    (Peking University Shenzhen Graduate School
    University of California)

  • Wei Qin

    (Peking University Shenzhen Graduate School)

  • Xiaochan Lu

    (Peking University Shenzhen Graduate School)

  • Jason Xu

    (University of California)

  • Haigen Huang

    (University of California)

  • Haipeng Bai

    (Peking University Shenzhen Graduate School)

  • Song Li

    (Peking University Shenzhen Graduate School)

  • Shuo Lin

    (Peking University Shenzhen Graduate School
    University of California)

Abstract

Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable “base editing” system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5′-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.

Suggested Citation

  • Yihan Zhang & Wei Qin & Xiaochan Lu & Jason Xu & Haigen Huang & Haipeng Bai & Song Li & Shuo Lin, 2017. "Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system," Nature Communications, Nature, vol. 8(1), pages 1-5, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00175-6
    DOI: 10.1038/s41467-017-00175-6
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    Cited by:

    1. Chengdong Zhang & Yuan Yang & Tao Qi & Yuening Zhang & Linghui Hou & Jingjing Wei & Jingcheng Yang & Leming Shi & Sang-Ging Ong & Hongyan Wang & Hui Wang & Bo Yu & Yongming Wang, 2023. "Prediction of base editor off-targets by deep learning," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Luke Hoberecht & Pirunthan Perampalam & Aaron Lun & Jean-Philippe Fortin, 2022. "A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies," Nature Communications, Nature, vol. 13(1), pages 1-20, December.

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