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A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging

Author

Listed:
  • Alexander L. Antaris

    (Stanford University)

  • Hao Chen

    (State Key Laboratory of Virology, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences
    Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University)

  • Shuo Diao

    (Stanford University)

  • Zhuoran Ma

    (Stanford University)

  • Zhe Zhang

    (Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University)

  • Shoujun Zhu

    (Stanford University)

  • Joy Wang

    (Stanford University)

  • Alexander X. Lozano

    (Stanford University)

  • Quli Fan

    (Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University)

  • Leila Chew

    (Stanford University)

  • Mark Zhu

    (Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University)

  • Kai Cheng

    (Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University)

  • Xuechuan Hong

    (State Key Laboratory of Virology, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences)

  • Hongjie Dai

    (Stanford University)

  • Zhen Cheng

    (Molecular Imaging Program at Stanford (MIPS), Bio-X Program, Canary Center at Stanford for Cancer Early Detection, Stanford University)

Abstract

Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with >1,000 nm emission suffer from low quantum yields that have limited temporal resolution and penetration depth. Here, we report tailoring the supramolecular assemblies of protein complexes with a sulfonated NIR-II organic dye (CH-4T) to produce a brilliant 110-fold increase in fluorescence, resulting in the highest quantum yield molecular fluorophore thus far. The bright molecular complex allowed for the fastest video-rate imaging in the second NIR window with ∼50-fold reduced exposure times at a fast 50 frames-per-second (FPS) capable of resolving mouse cardiac cycles. In addition, we demonstrate that the NIR-II molecular complexes are superior to clinically approved ICG for lymph node imaging deep within the mouse body.

Suggested Citation

  • Alexander L. Antaris & Hao Chen & Shuo Diao & Zhuoran Ma & Zhe Zhang & Shoujun Zhu & Joy Wang & Alexander X. Lozano & Quli Fan & Leila Chew & Mark Zhu & Kai Cheng & Xuechuan Hong & Hongjie Dai & Zhen , 2017. "A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15269
    DOI: 10.1038/ncomms15269
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    Cited by:

    1. Junjie Chen & Longqi Chen & Yinglong Wu & Yichang Fang & Fang Zeng & Shuizhu Wu & Yanli Zhao, 2021. "A H2O2-activatable nanoprobe for diagnosing interstitial cystitis and liver ischemia-reperfusion injury via multispectral optoacoustic tomography and NIR-II fluorescent imaging," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    2. Aiyan Ji & Hongyue Lou & Chunrong Qu & Wanglong Lu & Yifan Hao & Jiafeng Li & Yuyang Wu & Tonghang Chang & Hao Chen & Zhen Cheng, 2022. "Acceptor engineering for NIR-II dyes with high photochemical and biomedical performance," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Qing Zhang & Ruoyu Wang & Bowen Feng & Xiaoxia Zhong & Kostya (Ken) Ostrikov, 2021. "Photoluminescence mechanism of carbon dots: triggering high-color-purity red fluorescence emission through edge amino protonation," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

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