Author
Listed:
- Nicolas Tissot
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
- Jean-Antoine Lepesant
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
- Fred Bernard
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
- Kevin Legent
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
- Floris Bosveld
(Polarity, Institut Curie, CNRS UMR 3215, INSERM U934)
- Charlotte Martin
(Polarity, Institut Curie, CNRS UMR 3215, INSERM U934)
- Orestis Faklaris
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
- Yohanns Bellaïche
(Polarity, Institut Curie, CNRS UMR 3215, INSERM U934)
- Maïté Coppey
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
- Antoine Guichet
(Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité)
Abstract
Controlling nucleus localization is crucial for a variety of cellular functions. In the Drosophila oocyte, nuclear asymmetric positioning is essential for the reorganization of the microtubule (MT) network that controls the polarized transport of axis determinants. A combination of quantitative three-dimensional live imaging and laser ablation-mediated force analysis reveal that nuclear positioning is ensured with an unexpected level of robustness. We show that the nucleus is pushed to the oocyte antero-dorsal cortex by MTs and that its migration can proceed through distinct tracks. Centrosome-associated MTs favour one migratory route. In addition, the MT-associated protein Mud/NuMA that is asymmetrically localized in an Asp-dependent manner at the nuclear envelope hemisphere where MT nucleation is higher promotes a separate route. Our results demonstrate that centrosomes do not provide an obligatory driving force for nuclear movement, but together with Mud, contribute to the mechanisms that ensure the robustness of asymmetric nuclear positioning.
Suggested Citation
Nicolas Tissot & Jean-Antoine Lepesant & Fred Bernard & Kevin Legent & Floris Bosveld & Charlotte Martin & Orestis Faklaris & Yohanns Bellaïche & Maïté Coppey & Antoine Guichet, 2017.
"Distinct molecular cues ensure a robust microtubule-dependent nuclear positioning in the Drosophila oocyte,"
Nature Communications, Nature, vol. 8(1), pages 1-13, April.
Handle:
RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15168
DOI: 10.1038/ncomms15168
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15168. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.