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Metabolic labelling of the carbohydrate core in bacterial peptidoglycan and its applications

Author

Listed:
  • Hai Liang

    (University of Delaware)

  • Kristen E. DeMeester

    (University of Delaware)

  • Ching-Wen Hou

    (University of Delaware)

  • Michelle A. Parent

    (University of Delaware
    University of Delaware)

  • Jeffrey L. Caplan

    (University of Delaware
    Bioimaging Center, Delaware Biotechnology Institute)

  • Catherine L. Grimes

    (University of Delaware
    University of Delaware)

Abstract

Bacterial cells are surrounded by a polymer known as peptidoglycan (PG), which protects the cell from changes in osmotic pressure and small molecule insults. A component of this material, N-acetyl-muramic acid (NAM), serves as a core structural element for innate immune recognition of PG fragments. We report the synthesis of modifiable NAM carbohydrate derivatives and the installation of these building blocks into the backbone of Gram-positive and Gram-negative bacterial PG utilizing metabolic cell wall recycling and biosynthetic machineries. Whole cells are labelled via click chemistry and visualized using super-resolution microscopy, revealing higher resolution PG structural details and allowing the cell wall biosynthesis, as well as its destruction in immune cells, to be tracked. This study will assist in the future identification of mechanisms that the immune system uses to recognize bacteria, glean information about fundamental cell wall architecture and aid in the design of novel antibiotics.

Suggested Citation

  • Hai Liang & Kristen E. DeMeester & Ching-Wen Hou & Michelle A. Parent & Jeffrey L. Caplan & Catherine L. Grimes, 2017. "Metabolic labelling of the carbohydrate core in bacterial peptidoglycan and its applications," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15015
    DOI: 10.1038/ncomms15015
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    Cited by:

    1. Xiao Tian & Lingna Zheng & Changjiang Wang & Yida Han & Yujie Li & Tongxiao Cui & Jialin Liu & Chuanming Liu & Guogeng Jia & Lujie Yang & Yi Hsu & Chen Zeng & Lijun Ding & Chu Wang & Bo Cheng & Meng W, 2023. "Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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