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Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

Author

Listed:
  • Shicai Xu

    (Shandong Provincial Key Laboratory of Biophysics, College of Physics and Electronic Information, Dezhou University)

  • Jian Zhan

    (Institute for Glycomics and School of Information and Communication Technology, Griffith University)

  • Baoyuan Man

    (School of Physics and Electronics, Shandong Normal University)

  • Shouzhen Jiang

    (School of Physics and Electronics, Shandong Normal University)

  • Weiwei Yue

    (School of Physics and Electronics, Shandong Normal University)

  • Shoubao Gao

    (School of Physics and Electronics, Shandong Normal University)

  • Chengang Guo

    (Shandong Provincial Key Laboratory of Biophysics, College of Physics and Electronic Information, Dezhou University)

  • Hanping Liu

    (Shandong Provincial Key Laboratory of Biophysics, College of Physics and Electronic Information, Dezhou University)

  • Zhenhua Li

    (Shandong Provincial Key Laboratory of Biophysics, College of Physics and Electronic Information, Dezhou University)

  • Jihua Wang

    (Shandong Provincial Key Laboratory of Biophysics, College of Physics and Electronic Information, Dezhou University)

  • Yaoqi Zhou

    (Shandong Provincial Key Laboratory of Biophysics, College of Physics and Electronic Information, Dezhou University
    Institute for Glycomics and School of Information and Communication Technology, Griffith University)

Abstract

Reliable determination of binding kinetics and affinity of DNA hybridization and single-base mismatches plays an essential role in systems biology, personalized and precision medicine. The standard tools are optical-based sensors that are difficult to operate in low cost and to miniaturize for high-throughput measurement. Biosensors based on nanowire field-effect transistors have been developed, but reliable and cost-effective fabrication remains a challenge. Here, we demonstrate that a graphene single-crystal domain patterned into multiple channels can measure time- and concentration-dependent DNA hybridization kinetics and affinity reliably and sensitively, with a detection limit of 10 pM for DNA. It can distinguish single-base mutations quantitatively in real time. An analytical model is developed to estimate probe density, efficiency of hybridization and the maximum sensor response. The results suggest a promising future for cost-effective, high-throughput screening of drug candidates, genetic variations and disease biomarkers by using an integrated, miniaturized, all-electrical multiplexed, graphene-based DNA array.

Suggested Citation

  • Shicai Xu & Jian Zhan & Baoyuan Man & Shouzhen Jiang & Weiwei Yue & Shoubao Gao & Chengang Guo & Hanping Liu & Zhenhua Li & Jihua Wang & Yaoqi Zhou, 2017. "Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor," Nature Communications, Nature, vol. 8(1), pages 1-10, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14902
    DOI: 10.1038/ncomms14902
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    Cited by:

    1. Hu Li & Huarui Gong & Tsz Hung Wong & Jingkun Zhou & Yuqiong Wang & Long Lin & Ying Dou & Huiling Jia & Xingcan Huang & Zhan Gao & Rui Shi & Ya Huang & Zhenlin Chen & Wooyoung PARK & Ji Yu Li & Hongwe, 2023. "Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and severity evaluation," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Jack Hu & Fareeha Safir & Kai Chang & Sahil Dagli & Halleh B. Balch & John M. Abendroth & Jefferson Dixon & Parivash Moradifar & Varun Dolia & Malaya K. Sahoo & Benjamin A. Pinsky & Stefanie S. Jeffre, 2023. "Rapid genetic screening with high quality factor metasurfaces," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

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