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Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses

Author

Listed:
  • Daigo Inoue

    (Centre for Organismal Studies (COS), Heidelberg University)

  • Manuel Stemmer

    (Centre for Organismal Studies (COS), Heidelberg University)

  • Thomas Thumberger

    (Centre for Organismal Studies (COS), Heidelberg University)

  • Thomas Ruppert

    (Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance)

  • Felix Bärenz

    (Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance
    Present address: Sanofi Aventis Deutschland GmbH, Industriepark Hoechst, 65926 Frankfurt, Germany)

  • Joachim Wittbrodt

    (Centre for Organismal Studies (COS), Heidelberg University)

  • Oliver J. Gruss

    (Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance
    Institute of Genetics, University of Bonn)

Abstract

The assembly of the first centrosome occurs upon fertilisation when male centrioles recruit pericentriolar material (PCM) from the egg cytoplasm. The mechanisms underlying the proper assembly of centrosomes during early embryogenesis remain obscure. We identify Wdr8 as a novel maternally essential protein that is required for centrosome assembly during embryonic mitoses of medaka (Oryzias latipes). By CRISPR–Cas9-mediated knockout, maternal/zygotic Wdr8-null (m/zWdr8−/−) blastomeres exhibit severe defects in centrosome structure that lead to asymmetric division, multipolar mitotic spindles and chromosome alignment errors. Via its WD40 domains, Wdr8 interacts with the centriolar satellite protein SSX2IP. Combining targeted gene knockout and in vivo reconstitution of the maternally essential Wdr8–SSX2IP complex reveals an essential link between maternal centrosome proteins and the stability of the zygotic genome for accurate vertebrate embryogenesis. Our approach provides a way of distinguishing maternal from paternal effects in early embryos and should contribute to understanding molecular defects in human infertility.

Suggested Citation

  • Daigo Inoue & Manuel Stemmer & Thomas Thumberger & Thomas Ruppert & Felix Bärenz & Joachim Wittbrodt & Oliver J. Gruss, 2017. "Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses," Nature Communications, Nature, vol. 8(1), pages 1-12, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14090
    DOI: 10.1038/ncomms14090
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    Cited by:

    1. Ai Kiyomitsu & Toshiya Nishimura & Shiang Jyi Hwang & Satoshi Ansai & Masato T. Kanemaki & Minoru Tanaka & Tomomi Kiyomitsu, 2024. "Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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