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Two pathways regulate cortical granule translocation to prevent polyspermy in mouse oocytes

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Listed:
  • Liam P. Cheeseman

    (Medical Research Council Laboratory of Molecular Biology)

  • Jérôme Boulanger

    (Medical Research Council Laboratory of Molecular Biology)

  • Lisa M. Bond

    (Medical Research Council Laboratory of Molecular Biology)

  • Melina Schuh

    (Medical Research Council Laboratory of Molecular Biology
    Max Planck Institute for Biophysical Chemistry)

Abstract

An egg must be fertilized by a single sperm only. To prevent polyspermy, the zona pellucida, a structure that surrounds mammalian eggs, becomes impermeable upon fertilization, preventing the entry of further sperm. The structural changes in the zona upon fertilization are driven by the exocytosis of cortical granules. These translocate from the oocyte’s centre to the plasma membrane during meiosis. However, very little is known about the mechanism of cortical granule translocation. Here we investigate cortical granule transport and dynamics in live mammalian oocytes by using Rab27a as a marker. We show that two separate mechanisms drive their transport: myosin Va-dependent movement along actin filaments, and an unexpected vesicle hitchhiking mechanism by which cortical granules bind to Rab11a vesicles powered by myosin Vb. Inhibiting cortical granule translocation severely impaired the block to sperm entry, suggesting that translocation defects could contribute to miscarriages that are caused by polyspermy.

Suggested Citation

  • Liam P. Cheeseman & Jérôme Boulanger & Lisa M. Bond & Melina Schuh, 2016. "Two pathways regulate cortical granule translocation to prevent polyspermy in mouse oocytes," Nature Communications, Nature, vol. 7(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13726
    DOI: 10.1038/ncomms13726
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    Cited by:

    1. John W. Oller & Christopher A. Shaw, 2019. "From Superficial Damage to Invasion of the Nucleosome: Ranking of Morbidities by the Biosemiotic Depth Hypothesis," International Journal of Sciences, Office ijSciences, vol. 8(06), pages 51-73, June.

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