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Five colour variants of bright luminescent protein for real-time multicolour bioimaging

Author

Listed:
  • Kazushi Suzuki

    (Graduate School of Engineering, Osaka University)

  • Taichi Kimura

    (School of Engineering, Osaka University)

  • Hajime Shinoda

    (Graduate School of Engineering, Osaka University)

  • Guirong Bai

    (The Institute of Scientific and Industrial Research, Osaka University)

  • Matthew J. Daniels

    (BHF Centre for Regenerative Medicine)

  • Yoshiyuki Arai

    (Graduate School of Engineering, Osaka University
    School of Engineering, Osaka University
    The Institute of Scientific and Industrial Research, Osaka University)

  • Masahiro Nakano

    (Graduate School of Engineering, Osaka University
    School of Engineering, Osaka University
    The Institute of Scientific and Industrial Research, Osaka University)

  • Takeharu Nagai

    (Graduate School of Engineering, Osaka University
    School of Engineering, Osaka University
    The Institute of Scientific and Industrial Research, Osaka University)

Abstract

Luminescence imaging has gained attention as a promising bio-imaging modality in situations where fluorescence imaging cannot be applied. However, wider application to multicolour and dynamic imaging is limited by the lack of bright luminescent proteins with emissions across the visible spectrum. Here we report five new spectral variants of the bright luminescent protein, enhanced Nano-lantern (eNL), made by concatenation of the brightest luciferase, NanoLuc, with various colour hues of fluorescent proteins. eNLs allow five-colour live-cell imaging, as well as detection of single protein complexes and even single molecules. We also develop an eNL-based Ca2+ indicator with a 500% signal change, which can image spontaneous Ca2+ dynamics in cardiomyocyte and neural cell models. These eNL probes facilitate not only multicolour imaging in living cells but also sensitive imaging of a wide repertoire of proteins, even at very low expression levels.

Suggested Citation

  • Kazushi Suzuki & Taichi Kimura & Hajime Shinoda & Guirong Bai & Matthew J. Daniels & Yoshiyuki Arai & Masahiro Nakano & Takeharu Nagai, 2016. "Five colour variants of bright luminescent protein for real-time multicolour bioimaging," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13718
    DOI: 10.1038/ncomms13718
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    Cited by:

    1. Xiaodong Tian & Yiyu Zhang & Xinyu Li & Ying Xiong & Tianchen Wu & Hui-Wang Ai, 2022. "A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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