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A modular platform for bioluminescent RNA tracking

Author

Listed:
  • Lila P. Halbers

    (University of California, Irvine)

  • Kyle H. Cole

    (University of California, Irvine)

  • Kevin K. Ng

    (University of California, Irvine)

  • Erin B. Fuller

    (University of California, Irvine)

  • Christelle E. T. Chan

    (University of California, Irvine)

  • Chelsea Callicoatte

    (University of California, Irvine)

  • Mariajose Metcalfe

    (University of California, Irvine)

  • Claire C. Chen

    (University of California, Irvine)

  • Ahfnan A. Barhoosh

    (University of California, Irvine)

  • Edison Reid-McLaughlin

    (University of California, Irvine)

  • Alexandra D. Kent

    (University of California, Irvine)

  • Zachary R. Torrey

    (University of California, Irvine)

  • Oswald Steward

    (University of California, Irvine)

  • Andrej Lupták

    (University of California, Irvine
    University of California, Irvine
    University of California, Irvine)

  • Jennifer A. Prescher

    (University of California, Irvine
    University of California, Irvine
    University of California, Irvine)

Abstract

A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs. The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription. Robust photon production is observed for RNA targets both in cells and in live animals. Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats. Overall, this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale.

Suggested Citation

  • Lila P. Halbers & Kyle H. Cole & Kevin K. Ng & Erin B. Fuller & Christelle E. T. Chan & Chelsea Callicoatte & Mariajose Metcalfe & Claire C. Chen & Ahfnan A. Barhoosh & Edison Reid-McLaughlin & Alexan, 2024. "A modular platform for bioluminescent RNA tracking," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54263-5
    DOI: 10.1038/s41467-024-54263-5
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    References listed on IDEAS

    as
    1. Kazushi Suzuki & Taichi Kimura & Hajime Shinoda & Guirong Bai & Matthew J. Daniels & Yoshiyuki Arai & Masahiro Nakano & Takeharu Nagai, 2016. "Five colour variants of bright luminescent protein for real-time multicolour bioimaging," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
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