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PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation

Author

Listed:
  • Yuji Nagai

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Erika Kikuchi

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Walter Lerchner

    (Laboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health)

  • Ken-ichi Inoue

    (Systems Neuroscience Section, Primate Research Institute, Kyoto University)

  • Bin Ji

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Mark A. G. Eldridge

    (Laboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health)

  • Hiroyuki Kaneko

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Yasuyuki Kimura

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Arata Oh-Nishi

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Yukiko Hori

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Yoko Kato

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Toshiyuki Hirabayashi

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Atsushi Fujimoto

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Katsushi Kumata

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Ming-Rong Zhang

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Ichio Aoki

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Tetsuya Suhara

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Makoto Higuchi

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

  • Masahiko Takada

    (Systems Neuroscience Section, Primate Research Institute, Kyoto University)

  • Barry J. Richmond

    (Laboratory of Neuropsychology, National Institute of Mental Health, National Institutes of Health)

  • Takafumi Minamimoto

    (National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology)

Abstract

The rostromedial caudate (rmCD) of primates is thought to contribute to reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) to repeatedly and non-invasively inactivate rmCD of macaque monkeys. We inject an adeno-associated viral vector expressing the inhibitory DREADD, hM4Di, into the rmCD bilaterally. To visualize DREADD expression in vivo, we develop a non-invasive imaging method using positron emission tomography (PET). PET imaging provides information critical for successful chemogenetic silencing during experiments, in this case the location and level of hM4Di expression, and the relationship between agonist dose and hM4Di receptor occupancy. Here we demonstrate that inactivating bilateral rmCD through activation of hM4Di produces a significant and reproducible loss of sensitivity to reward value in monkeys. Thus, the rmCD is involved in making normal judgments about the value of reward.

Suggested Citation

  • Yuji Nagai & Erika Kikuchi & Walter Lerchner & Ken-ichi Inoue & Bin Ji & Mark A. G. Eldridge & Hiroyuki Kaneko & Yasuyuki Kimura & Arata Oh-Nishi & Yukiko Hori & Yoko Kato & Toshiyuki Hirabayashi & At, 2016. "PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation," Nature Communications, Nature, vol. 7(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13605
    DOI: 10.1038/ncomms13605
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    Cited by:

    1. Naohisa Miyakawa & Yuji Nagai & Yukiko Hori & Koki Mimura & Asumi Orihara & Kei Oyama & Takeshi Matsuo & Ken-ichi Inoue & Takafumi Suzuki & Toshiyuki Hirabayashi & Tetsuya Suhara & Masahiko Takada & M, 2023. "Chemogenetic attenuation of cortical seizures in nonhuman primates," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Masahiro Mitsuhashi & Reona Yamaguchi & Toshinari Kawasaki & Satoko Ueno & Yiping Sun & Kaoru Isa & Jun Takahashi & Kenta Kobayashi & Hirotaka Onoe & Ryosuke Takahashi & Tadashi Isa, 2024. "Stage-dependent role of interhemispheric pathway for motor recovery in primates," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Toshiyuki Hirabayashi & Yuji Nagai & Yuki Hori & Yukiko Hori & Kei Oyama & Koki Mimura & Naohisa Miyakawa & Haruhiko Iwaoki & Ken-ichi Inoue & Tetsuya Suhara & Masahiko Takada & Makoto Higuchi & Takaf, 2024. "Multiscale chemogenetic dissection of fronto-temporal top-down regulation for object memory in primates," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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