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A redox mechanism underlying nucleolar stress sensing by nucleophosmin

Author

Listed:
  • Kai Yang

    (Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine)

  • Ming Wang

    (Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine)

  • Yuzheng Zhao

    (Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology)

  • Xuxu Sun

    (Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine)

  • Yi Yang

    (Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology)

  • Xie Li

    (Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology)

  • Aiwu Zhou

    (Shanghai Jiao Tong University School of Medicine)

  • Huilin Chu

    (Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine)

  • Hu Zhou

    (Shanghai Institute of Materia Medica)

  • Jianrong Xu

    (Shanghai Jiao Tong University School of Medicine)

  • Mian Wu

    (School of Life Sciences, University of Science and Technology of China)

  • Jie Yang

    (Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine)

  • Jing Yi

    (Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine)

Abstract

The nucleolus has been recently described as a stress sensor. The nucleoplasmic translocation of nucleolar protein nucleophosmin (NPM1) is a hallmark of nucleolar stress; however, the causes of this translocation and its connection to p53 activation are unclear. Using single live-cell imaging and the redox biosensors, we demonstrate that nucleolar oxidation is a general response to various cellular stresses. During nucleolar oxidation, NPM1 undergoes S-glutathionylation on cysteine 275, which triggers the dissociation of NPM1 from nucleolar nucleic acids. The C275S mutant NPM1, unable to be glutathionylated, remains in the nucleolus under nucleolar stress. Compared with wild-type NPM1 that can disrupt the p53–HDM2 interaction, the C275S mutant greatly compromises the activation of p53, highlighting that nucleoplasmic translocation of NPM1 is a prerequisite for stress-induced activation of p53. This study elucidates a redox mechanism for the nucleolar stress sensing and may help the development of therapeutic strategies.

Suggested Citation

  • Kai Yang & Ming Wang & Yuzheng Zhao & Xuxu Sun & Yi Yang & Xie Li & Aiwu Zhou & Huilin Chu & Hu Zhou & Jianrong Xu & Mian Wu & Jie Yang & Jing Yi, 2016. "A redox mechanism underlying nucleolar stress sensing by nucleophosmin," Nature Communications, Nature, vol. 7(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13599
    DOI: 10.1038/ncomms13599
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    Cited by:

    1. Tuo Ji & Lihua Zheng & Jiale Wu & Mei Duan & Qianwen Liu & Peng Liu & Chen Shen & Jinling Liu & Qinyi Ye & Jiangqi Wen & Jiangli Dong & Tao Wang, 2023. "The thioesterase APT1 is a bidirectional-adjustment redox sensor," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. K. A. Gajewska & H. Lescesen & M. Ramialison & K. M. Wagstaff & D. A. Jans, 2021. "Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

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