IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms12896.html
   My bibliography  Save this article

A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors

Author

Listed:
  • Stefania Averaimo

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
    CNRS, UMR_7210
    INSERM, UMR_S 968)

  • Ahlem Assali

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 839
    INSERM UMR_S 839
    Institut du Fer à Moulin)

  • Oriol Ros

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
    CNRS, UMR_7210
    INSERM, UMR_S 968)

  • Sandrine Couvet

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
    CNRS, UMR_7210
    INSERM, UMR_S 968)

  • Yvrick Zagar

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
    CNRS, UMR_7210
    INSERM, UMR_S 968)

  • Ioana Genescu

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
    CNRS, UMR_7210
    INSERM, UMR_S 968)

  • Alexandra Rebsam

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 839
    INSERM UMR_S 839
    Institut du Fer à Moulin)

  • Xavier Nicol

    (Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
    CNRS, UMR_7210
    INSERM, UMR_S 968)

Abstract

The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and are required for shaping the nervous system connectivity in vivo remains controversial. Here, we report a role for lipid rafts in generating domains of local cAMP signalling in axonal growth cones downstream of ephrin-A repulsive guidance cues. Ephrin-A-dependent retraction of retinal ganglion cell axons involves cAMP signalling restricted to the vicinity of lipid rafts and is independent of cAMP modulation outside of this microdomain. cAMP modulation near lipid rafts controls the pruning of ectopic axonal branches of retinal ganglion cells in vivo, a process requiring intact ephrin-A signalling. Together, our findings indicate that lipid rafts structure the subcellular organization of intracellular cAMP signalling shaping axonal arbors during the nervous system development.

Suggested Citation

  • Stefania Averaimo & Ahlem Assali & Oriol Ros & Sandrine Couvet & Yvrick Zagar & Ioana Genescu & Alexandra Rebsam & Xavier Nicol, 2016. "A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors," Nature Communications, Nature, vol. 7(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12896
    DOI: 10.1038/ncomms12896
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms12896
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms12896?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Léa Ripoll & Yong Li & Carmen W. Dessauer & Mark Zastrow, 2024. "Spatial organization of adenylyl cyclase and its impact on dopamine signaling in neurons," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Melody Atkins & Maud Wurmser & Michèle Darmon & Fiona Roche & Xavier Nicol & Christine Métin, 2023. "CXCL12 targets the primary cilium cAMP/cGMP ratio to regulate cell polarity during migration," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12896. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.