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Eigenspectra optoacoustic tomography achieves quantitative blood oxygenation imaging deep in tissues

Author

Listed:
  • Stratis Tzoumas

    (Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München
    Chair for Biological Imaging, Technische Universität München)

  • Antonio Nunes

    (Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München
    Present address: Bluepharma-Indústria Farmacêutica SA, S. Martinho do Bispo, Coimbra 3045-016, Portugal.)

  • Ivan Olefir

    (Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München)

  • Stefan Stangl

    (Klinikum rechts der Isar, Technische Universität München)

  • Panagiotis Symvoulidis

    (Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München
    Chair for Biological Imaging, Technische Universität München)

  • Sarah Glasl

    (Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München
    Chair for Biological Imaging, Technische Universität München)

  • Christine Bayer

    (Klinikum rechts der Isar, Technische Universität München)

  • Gabriele Multhoff

    (Klinikum rechts der Isar, Technische Universität München
    CCG—Innate immunity in Tumor Biology, Helmholtz Zentrum München)

  • Vasilis Ntziachristos

    (Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München
    Chair for Biological Imaging, Technische Universität München)

Abstract

Light propagating in tissue attains a spectrum that varies with location due to wavelength-dependent fluence attenuation, an effect that causes spectral corruption. Spectral corruption has limited the quantification accuracy of optical and optoacoustic spectroscopic methods, and impeded the goal of imaging blood oxygen saturation (sO2) deep in tissues; a critical goal for the assessment of oxygenation in physiological processes and disease. Here we describe light fluence in the spectral domain and introduce eigenspectra multispectral optoacoustic tomography (eMSOT) to account for wavelength-dependent light attenuation, and estimate blood sO2 within deep tissue. We validate eMSOT in simulations, phantoms and animal measurements and spatially resolve sO2 in muscle and tumours, validating our measurements with histology data. eMSOT shows substantial sO2 accuracy enhancement over previous optoacoustic methods, potentially serving as a valuable tool for imaging tissue pathophysiology.

Suggested Citation

  • Stratis Tzoumas & Antonio Nunes & Ivan Olefir & Stefan Stangl & Panagiotis Symvoulidis & Sarah Glasl & Christine Bayer & Gabriele Multhoff & Vasilis Ntziachristos, 2016. "Eigenspectra optoacoustic tomography achieves quantitative blood oxygenation imaging deep in tissues," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12121
    DOI: 10.1038/ncomms12121
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    Cited by:

    1. Xosé Luís Deán-Ben & Justine Robin & Daniil Nozdriukhin & Ruiqing Ni & Jim Zhao & Chaim Glück & Jeanne Droux & Juan Sendón-Lago & Zhenyue Chen & Quanyu Zhou & Bruno Weber & Susanne Wegener & Anxo Vida, 2023. "Deep optoacoustic localization microangiography of ischemic stroke in mice," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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