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Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment

Author

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  • Louie Wurch

    (Oak Ridge National Laboratory
    University of Tennessee
    Present address: Department of Biology, James Madison University, Harrisonburg, Virginia 22807, USA)

  • Richard J. Giannone

    (Oak Ridge National Laboratory)

  • Bernard S. Belisle

    (Oak Ridge National Laboratory
    University of Tennessee)

  • Carolyn Swift

    (Oak Ridge National Laboratory
    University of Tennessee)

  • Sagar Utturkar

    (Oak Ridge National Laboratory)

  • Robert L. Hettich

    (Oak Ridge National Laboratory
    University of Tennessee)

  • Anna-Louise Reysenbach

    (Portland State University)

  • Mircea Podar

    (Oak Ridge National Laboratory
    University of Tennessee)

Abstract

Biological features can be inferred, based on genomic data, for many microbial lineages that remain uncultured. However, cultivation is important for characterizing an organism’s physiology and testing its genome-encoded potential. Here we use single-cell genomics to infer cultivation conditions for the isolation of an ectosymbiotic Nanoarchaeota (‘Nanopusillus acidilobi’) and its host (Acidilobus, a crenarchaeote) from a terrestrial geothermal environment. The cells of ‘Nanopusillus’ are among the smallest known cellular organisms (100–300 nm). They appear to have a complete genetic information processing machinery, but lack almost all primary biosynthetic functions as well as respiration and ATP synthesis. Genomic and proteomic comparison with its distant relative, the marine Nanoarchaeum equitans illustrate an ancient, common evolutionary history of adaptation of the Nanoarchaeota to ectosymbiosis, so far unique among the Archaea.

Suggested Citation

  • Louie Wurch & Richard J. Giannone & Bernard S. Belisle & Carolyn Swift & Sagar Utturkar & Robert L. Hettich & Anna-Louise Reysenbach & Mircea Podar, 2016. "Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12115
    DOI: 10.1038/ncomms12115
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    Cited by:

    1. Su Ding & Joshua N. Hamm & Nicole J. Bale & Jaap S. Sinninghe Damsté & Anja Spang, 2024. "Selective lipid recruitment by an archaeal DPANN symbiont from its host," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Susanne Krause & Sabrina Gfrerer & Andriko Kügelgen & Carsten Reuse & Nina Dombrowski & Laura Villanueva & Boyke Bunk & Cathrin Spröer & Thomas R. Neu & Ute Kuhlicke & Kerstin Schmidt-Hohagen & Karste, 2022. "The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Matthew D. Johnson & Doulin C. Shepherd & Hiroyuki D. Sakai & Manasi Mudaliyar & Arun Prasad Pandurangan & Francesca L. Short & Paul D. Veith & Nichollas E. Scott & Norio Kurosawa & Debnath Ghosal, 2024. "Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    4. Joshua N. Hamm & Yan Liao & Andriko Kügelgen & Nina Dombrowski & Evan Landers & Christopher Brownlee & Emma M. V. Johansson & Renee M. Whan & Matthew A. B. Baker & Buzz Baum & Tanmay A. M. Bharat & Ia, 2024. "The parasitic lifestyle of an archaeal symbiont," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Stilianos Louca & Florent Mazel & Michael Doebeli & Laura Wegener Parfrey, 2019. "A census-based estimate of Earth's bacterial and archaeal diversity," PLOS Biology, Public Library of Science, vol. 17(2), pages 1-30, February.

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