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Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells

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Listed:
  • Congping Lin

    (School of Biosciences, University of Exeter
    Mathematics, University of Exeter)

  • Martin Schuster

    (School of Biosciences, University of Exeter)

  • Sofia Cunha Guimaraes

    (School of Biosciences, University of Exeter
    Present address: Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.)

  • Peter Ashwin

    (Mathematics, University of Exeter)

  • Michael Schrader

    (School of Biosciences, University of Exeter)

  • Jeremy Metz

    (School of Biosciences, University of Exeter)

  • Christian Hacker

    (School of Biosciences, University of Exeter)

  • Sarah Jane Gurr

    (School of Biosciences, University of Exeter)

  • Gero Steinberg

    (School of Biosciences, University of Exeter)

Abstract

Even distribution of peroxisomes (POs) and lipid droplets (LDs) is critical to their role in lipid and reactive oxygen species homeostasis. How even distribution is achieved remains elusive, but diffusive motion and directed motility may play a role. Here we show that in the fungus Ustilago maydis ∼95% of POs and LDs undergo diffusive motions. These movements require ATP and involve bidirectional early endosome motility, indicating that microtubule-associated membrane trafficking enhances diffusion of organelles. When early endosome transport is abolished, POs and LDs drift slowly towards the growing cell end. This pole-ward drift is facilitated by anterograde delivery of secretory cargo to the cell tip by myosin-5. Modelling reveals that microtubule-based directed transport and active diffusion support distribution, mobility and mixing of POs. In mammalian COS-7 cells, microtubules and F-actin also counteract each other to distribute POs. This highlights the importance of opposing cytoskeletal forces in organelle positioning in eukaryotes.

Suggested Citation

  • Congping Lin & Martin Schuster & Sofia Cunha Guimaraes & Peter Ashwin & Michael Schrader & Jeremy Metz & Christian Hacker & Sarah Jane Gurr & Gero Steinberg, 2016. "Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells," Nature Communications, Nature, vol. 7(1), pages 1-14, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11814
    DOI: 10.1038/ncomms11814
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    Cited by:

    1. Stephen Williams & Raphaël Jeanneret & Idan Tuval & Marco Polin, 2022. "Confinement-induced accumulation and de-mixing of microscopic active-passive mixtures," Nature Communications, Nature, vol. 13(1), pages 1-8, December.

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