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Octanol-assisted liposome assembly on chip

Author

Listed:
  • Siddharth Deshpande

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Yaron Caspi

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Anna E. C. Meijering

    (Kavli Institute of Nanoscience, Delft University of Technology)

  • Cees Dekker

    (Kavli Institute of Nanoscience, Delft University of Technology)

Abstract

Liposomes are versatile supramolecular assemblies widely used in basic and applied sciences. Here we present a novel microfluidics-based method, octanol-assisted liposome assembly (OLA), to form monodisperse, cell-sized (5–20 μm), unilamellar liposomes with excellent encapsulation efficiency. Akin to bubble blowing, an inner aqueous phase and a surrounding lipid-carrying 1-octanol phase is pinched off by outer fluid streams. Such hydrodynamic flow focusing results in double-emulsion droplets that spontaneously develop a side-connected 1-octanol pocket. Owing to interfacial energy minimization, the pocket splits off to yield fully assembled solvent-free liposomes within minutes. This solves the long-standing fundamental problem of prolonged presence of residual oil in the liposome bilayer. We demonstrate the unilamellarity of liposomes with functional α-haemolysin protein pores in the membrane and validate the biocompatibility by inner leaflet localization of bacterial divisome proteins (FtsZ and ZipA). OLA offers a versatile platform for future analytical tools, delivery systems, nanoreactors and synthetic cells.

Suggested Citation

  • Siddharth Deshpande & Yaron Caspi & Anna E. C. Meijering & Cees Dekker, 2016. "Octanol-assisted liposome assembly on chip," Nature Communications, Nature, vol. 7(1), pages 1-9, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10447
    DOI: 10.1038/ncomms10447
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    Cited by:

    1. Jorik Waeterschoot & Willemien Gosselé & Špela Lemež & Xavier Casadevall i Solvas, 2024. "Artificial cells for in vivo biomedical applications through red blood cell biomimicry," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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