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Large-scale inference of protein tissue origin in gram-positive sepsis plasma using quantitative targeted proteomics

Author

Listed:
  • Erik Malmström

    (Lund, Lund University)

  • Ola Kilsgård

    (Lund, Lund University)

  • Simon Hauri

    (Lund, Lund University)

  • Emanuel Smeds

    (Lund, Lund University)

  • Heiko Herwald

    (Lund, Lund University)

  • Lars Malmström

    (S3IT, University of Zurich)

  • Johan Malmström

    (Lund, Lund University)

Abstract

The plasma proteome is highly dynamic and variable, composed of proteins derived from surrounding tissues and cells. To investigate the complex processes that control the composition of the plasma proteome, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in murine plasma. The strategy relies on the construction of a comprehensive protein tissue atlas from cells and highly vascularized organs using shotgun mass spectrometry. The protein tissue atlas was transformed to a spectral library for highly reproducible quantification of tissue-specific proteins directly in plasma using SWATH-like data-independent mass spectrometry analysis. We show that the method can determine drastic changes of tissue-specific protein profiles in blood plasma from mouse animal models with sepsis. The strategy can be extended to several other species advancing our understanding of the complex processes that contribute to the plasma proteome dynamics.

Suggested Citation

  • Erik Malmström & Ola Kilsgård & Simon Hauri & Emanuel Smeds & Heiko Herwald & Lars Malmström & Johan Malmström, 2016. "Large-scale inference of protein tissue origin in gram-positive sepsis plasma using quantitative targeted proteomics," Nature Communications, Nature, vol. 7(1), pages 1-10, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10261
    DOI: 10.1038/ncomms10261
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    Cited by:

    1. Tirthankar Mohanty & Christofer A. Q. Karlsson & Yashuan Chao & Erik Malmström & Eleni Bratanis & Andrietta Grentzmann & Martina Mørch & Victor Nizet & Lars Malmström & Adam Linder & Oonagh Shannon & , 2023. "A pharmacoproteomic landscape of organotypic intervention responses in Gram-negative sepsis," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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