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Protein-targeted corona phase molecular recognition

Author

Listed:
  • Gili Bisker

    (Massachusetts Institute of Technology)

  • Juyao Dong

    (Massachusetts Institute of Technology)

  • Hoyoung D. Park

    (Massachusetts Institute of Technology)

  • Nicole M. Iverson

    (University of Nebraska-Lincoln)

  • Jiyoung Ahn

    (Massachusetts Institute of Technology)

  • Justin T. Nelson

    (Massachusetts Institute of Technology)

  • Markita P. Landry

    (University of California Berkeley)

  • Sebastian Kruss

    (Institute for Physical Chemistry, Göttingen University)

  • Michael S. Strano

    (Massachusetts Institute of Technology)

Abstract

Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications.

Suggested Citation

  • Gili Bisker & Juyao Dong & Hoyoung D. Park & Nicole M. Iverson & Jiyoung Ahn & Justin T. Nelson & Markita P. Landry & Sebastian Kruss & Michael S. Strano, 2016. "Protein-targeted corona phase molecular recognition," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10241
    DOI: 10.1038/ncomms10241
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    Cited by:

    1. Simon Settele & C. Alexander Schrage & Sebastian Jung & Elena Michel & Han Li & Benjamin S. Flavel & A. Stephen K. Hashmi & Sebastian Kruss & Jana Zaumseil, 2024. "Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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