IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-45052-1.html
   My bibliography  Save this article

Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes

Author

Listed:
  • Simon Settele

    (Universität Heidelberg)

  • C. Alexander Schrage

    (Ruhr-Universität Bochum)

  • Sebastian Jung

    (Ruhr-Universität Bochum)

  • Elena Michel

    (Universität Heidelberg)

  • Han Li

    (Karlsruhe Institute of Technology
    University of Turku)

  • Benjamin S. Flavel

    (Karlsruhe Institute of Technology)

  • A. Stephen K. Hashmi

    (Universität Heidelberg
    King Abdulaziz University)

  • Sebastian Kruss

    (Ruhr-Universität Bochum
    Fraunhofer Institute for Microelectronic Circuits and Systems)

  • Jana Zaumseil

    (Universität Heidelberg)

Abstract

Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors.

Suggested Citation

  • Simon Settele & C. Alexander Schrage & Sebastian Jung & Elena Michel & Han Li & Benjamin S. Flavel & A. Stephen K. Hashmi & Sebastian Kruss & Jana Zaumseil, 2024. "Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45052-1
    DOI: 10.1038/s41467-024-45052-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-45052-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-45052-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Robert Nißler & Oliver Bader & Maria Dohmen & Sebastian G. Walter & Christine Noll & Gabriele Selvaggio & Uwe Groß & Sebastian Kruss, 2020. "Remote near infrared identification of pathogens with multiplexed nanosensors," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    2. Gili Bisker & Juyao Dong & Hoyoung D. Park & Nicole M. Iverson & Jiyoung Ahn & Justin T. Nelson & Markita P. Landry & Sebastian Kruss & Michael S. Strano, 2016. "Protein-targeted corona phase molecular recognition," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      More about this item

      Statistics

      Access and download statistics

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45052-1. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.