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Mussel adhesion is dictated by time-regulated secretion and molecular conformation of mussel adhesive proteins

Author

Listed:
  • Luigi Petrone

    (School of Materials Science and Engineering, Nanyang Technological University
    Centre for Biomimetic Sensor Science, Nanyang Technological University)

  • Akshita Kumar

    (Centre for Biomimetic Sensor Science, Nanyang Technological University
    School of Biological Sciences, Nanyang Technological University)

  • Clarinda N. Sutanto

    (School of Materials Science and Engineering, Nanyang Technological University
    Centre for Biomimetic Sensor Science, Nanyang Technological University)

  • Navinkumar J. Patil

    (Università della Calabria)

  • Srinivasaraghavan Kannan

    (Bioinformatics Institute A*Star)

  • Alagappan Palaniappan

    (School of Materials Science and Engineering, Nanyang Technological University
    Centre for Biomimetic Sensor Science, Nanyang Technological University)

  • Shahrouz Amini

    (School of Materials Science and Engineering, Nanyang Technological University
    Centre for Biomimetic Sensor Science, Nanyang Technological University)

  • Bruno Zappone

    (Consiglio Nazionale delle Ricerche, CNR-Nanotec, UOS Licryl-Cosenza)

  • Chandra Verma

    (School of Biological Sciences, Nanyang Technological University
    Bioinformatics Institute A*Star
    National University of Singapore)

  • Ali Miserez

    (School of Materials Science and Engineering, Nanyang Technological University
    Centre for Biomimetic Sensor Science, Nanyang Technological University
    School of Biological Sciences, Nanyang Technological University)

Abstract

Interfacial water constitutes a formidable barrier to strong surface bonding, hampering the development of water-resistant synthetic adhesives. Notwithstanding this obstacle, the Asian green mussel Perna viridis attaches firmly to underwater surfaces via a proteinaceous secretion (byssus). Extending beyond the currently known design principles of mussel adhesion, here we elucidate the precise time-regulated secretion of P. viridis mussel adhesive proteins. The vanguard 3,4-dihydroxy-L-phenylalanine (Dopa)-rich protein Pvfp-5 acts as an adhesive primer, overcoming repulsive hydration forces by displacing surface-bound water and generating strong surface adhesion. Using homology modelling and molecular dynamics simulations, we find that all mussel adhesive proteins are largely unordered, with Pvfp-5 adopting a disordered structure and elongated conformation whereby all Dopa residues reside on the protein surface. Time-regulated secretion and structural disorder of mussel adhesive proteins appear essential for optimizing extended nonspecific surface interactions and byssus’ assembly. Our findings reveal molecular-scale principles to help the development of wet-resistant adhesives.

Suggested Citation

  • Luigi Petrone & Akshita Kumar & Clarinda N. Sutanto & Navinkumar J. Patil & Srinivasaraghavan Kannan & Alagappan Palaniappan & Shahrouz Amini & Bruno Zappone & Chandra Verma & Ali Miserez, 2015. "Mussel adhesion is dictated by time-regulated secretion and molecular conformation of mussel adhesive proteins," Nature Communications, Nature, vol. 6(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9737
    DOI: 10.1038/ncomms9737
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    Cited by:

    1. Yuhe Shen & Rongxin Su & Dongzhao Hao & Xiaojian Xu & Meital Reches & Jiwei Min & Heng Chang & Tao Yu & Qing Li & Xiaoyu Zhang & Yuefei Wang & Yuefei Wang & Wei Qi, 2023. "Enzymatic polymerization of enantiomeric L−3,4-dihydroxyphenylalanine into films with enhanced rigidity and stability," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Yongchun Liu & Ke Li & Juanhua Tian & Aiting Gao & Lihua Tian & Hao Su & Shuting Miao & Fei Tao & Hao Ren & Qingmin Yang & Jing Cao & Peng Yang, 2023. "Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Qi Guo & Guijin Zou & Xuliang Qian & Shujun Chen & Huajian Gao & Jing Yu, 2022. "Hydrogen-bonds mediate liquid-liquid phase separation of mussel derived adhesive peptides," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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