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Stem and progenitor cell division kinetics during postnatal mouse mammary gland development

Author

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  • Rajshekhar R. Giraddi

    (Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge)

  • Mona Shehata

    (Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge)

  • Mercedes Gallardo

    (Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO))

  • Maria A. Blasco

    (Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO))

  • Benjamin D. Simons

    (Cavendish Laboratory, University of Cambridge
    The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
    Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge)

  • John Stingl

    (Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge)

Abstract

The cycling properties of mammary stem and progenitor cells is not well understood. To determine the division properties of these cells, we administered synthetic nucleosides for varying periods of time to mice at different stages of postnatal development and monitored the rate of uptake of these nucleosides in the different mammary cell compartments. Here we show that most cell division in the adult virgin gland is restricted to the oestrogen receptor-expressing luminal cell lineage. Our data also demonstrate that the oestrogen receptor-expressing, milk and basal cell subpopulations have telomere lengths and cell division kinetics that are not compatible with these cells being hierarchically organized; instead, our data indicate that in the adult homeostatic gland, each cell type is largely maintained by its own restricted progenitors. We also observe that transplantable stem cells are largely quiescent during oestrus, but are cycling during dioestrus when progesterone levels are high.

Suggested Citation

  • Rajshekhar R. Giraddi & Mona Shehata & Mercedes Gallardo & Maria A. Blasco & Benjamin D. Simons & John Stingl, 2015. "Stem and progenitor cell division kinetics during postnatal mouse mammary gland development," Nature Communications, Nature, vol. 6(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9487
    DOI: 10.1038/ncomms9487
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    Cited by:

    1. Maryam Ghaderi Najafabadi & G. Kenneth Gray & Li Ren Kong & Komal Gupta & David Perera & Huw Naylor & Joan S. Brugge & Ashok R. Venkitaraman & Mona Shehata, 2023. "A transcriptional response to replication stress selectively expands a subset of Brca2-mutant mammary epithelial cells," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Joseph G. Kern & Andrew M. Tilston-Lunel & Anthony Federico & Boting Ning & Amy Mueller & Grace B. Peppler & Eleni Stampouloglou & Nan Cheng & Randy L. Johnson & Marc E. Lenburg & Jennifer E. Beane & , 2022. "Inactivation of LATS1/2 drives luminal-basal plasticity to initiate basal-like mammary carcinomas," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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