IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8907.html
   My bibliography  Save this article

The photochemical mechanism of a B12-dependent photoreceptor protein

Author

Listed:
  • Roger J. Kutta

    (School of Chemistry, The University of Manchester, Oxford Road
    Photon Science Institute, The University of Manchester
    SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester)

  • Samantha J. O. Hardman

    (SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester
    Faculty of Life Sciences, The University of Manchester, Carys Bannister Building, Dover Street)

  • Linus O. Johannissen

    (SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester
    Faculty of Life Sciences, The University of Manchester, Carys Bannister Building, Dover Street)

  • Bruno Bellina

    (School of Chemistry, The University of Manchester, Oxford Road
    SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester)

  • Hanan L. Messiha

    (SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester
    Faculty of Life Sciences, The University of Manchester, Carys Bannister Building, Dover Street)

  • Juan Manuel Ortiz-Guerrero

    (Area of Genetics (Unidad Asociada al Instituto de Química Física–Consejo Superior de Investigaciones Científicas), Faculty of Biology, Universidad de Murcia)

  • Montserrat Elías-Arnanz

    (Area of Genetics (Unidad Asociada al Instituto de Química Física–Consejo Superior de Investigaciones Científicas), Faculty of Biology, Universidad de Murcia)

  • S. Padmanabhan

    (Instituto de Química Física ‘Rocasolano’, Consejo Superior de Investigaciones Científicas)

  • Perdita Barran

    (School of Chemistry, The University of Manchester, Oxford Road
    SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester)

  • Nigel S. Scrutton

    (SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester
    Faculty of Life Sciences, The University of Manchester, Carys Bannister Building, Dover Street)

  • Alex R. Jones

    (School of Chemistry, The University of Manchester, Oxford Road
    Photon Science Institute, The University of Manchester
    SYNBIOCHEM, Manchester Institute of Biotechnology, The University of Manchester)

Abstract

The coenzyme B12-dependent photoreceptor protein, CarH, is a bacterial transcriptional regulator that controls the biosynthesis of carotenoids in response to light. On binding of coenzyme B12 the monomeric apoprotein forms tetramers in the dark, which bind operator DNA thus blocking transcription. Under illumination the CarH tetramer dissociates, weakening its affinity for DNA and allowing transcription. The mechanism by which this occurs is unknown. Here we describe the photochemistry in CarH that ultimately triggers tetramer dissociation; it proceeds via a cob(III)alamin intermediate, which then forms a stable adduct with the protein. This pathway is without precedent and our data suggest it is independent of the radical chemistry common to both coenzyme B12 enzymology and its known photochemistry. It provides a mechanistic foundation for the emerging field of B12 photobiology and will serve to inform the development of a new class of optogenetic tool for the control of gene expression.

Suggested Citation

  • Roger J. Kutta & Samantha J. O. Hardman & Linus O. Johannissen & Bruno Bellina & Hanan L. Messiha & Juan Manuel Ortiz-Guerrero & Montserrat Elías-Arnanz & S. Padmanabhan & Perdita Barran & Nigel S. Sc, 2015. "The photochemical mechanism of a B12-dependent photoreceptor protein," Nature Communications, Nature, vol. 6(1), pages 1-11, November.
  • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8907
    DOI: 10.1038/ncomms8907
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8907
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/ncomms8907?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Harshwardhan Poddar & Ronald Rios-Santacruz & Derren J. Heyes & Muralidharan Shanmugam & Adam Brookfield & Linus O. Johannissen & Colin W. Levy & Laura N. Jeffreys & Shaowei Zhang & Michiyo Sakuma & J, 2023. "Redox driven B12-ligand switch drives CarH photoresponse," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Shaowei Zhang & Laura N. Jeffreys & Harshwardhan Poddar & Yuqi Yu & Chuanyang Liu & Kaylee Patel & Linus O. Johannissen & Lingyun Zhu & Matthew J. Cliff & Cunyu Yan & Giorgio Schirò & Martin Weik & Mi, 2024. "Photocobilins integrate B12 and bilin photochemistry for enzyme control," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8907. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.