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Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling

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  • Hongwei Jing

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xiaolu Yang

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jian Zhang

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Xuehui Liu

    (Institute of Biophysics, Chinese Academy of Sciences)

  • Huakun Zheng

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Guojun Dong

    (State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences)

  • Jinqiang Nian

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Jian Feng

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Bin Xia

    (College of Life Sciences, Peking University)

  • Qian Qian

    (State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences)

  • Jiayang Li

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Jianru Zuo

    (State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

Abstract

In plants, auxin signalling is initiated by the auxin-promoted interaction between the auxin receptor TIR1, an E3 ubiquitin ligase, and the Aux/IAA transcriptional repressors, which are subsequently degraded by the proteasome. Gain-of-function mutations in the highly conserved domain II of Aux/IAAs abolish the TIR1–Aux/IAA interaction and thus cause an auxin-resistant phenotype. Here we show that peptidyl-prolyl isomerization of rice OsIAA11 catalysed by LATERAL ROOTLESS2 (LRT2), a cyclophilin-type peptidyl-prolyl cis/trans isomerase, directly regulates the stability of OsIAA11. NMR spectroscopy reveals that LRT2 efficiently catalyses the cis/trans isomerization of OsIAA11. The lrt2 mutation reduces OsTIR1–OsIAA11 interaction and consequently causes the accumulation of a higher level of OsIAA11 protein. Moreover, knockdown of the OsIAA11 expression partially rescues the lrt2 mutant phenotype in lateral root development. Together, these results illustrate cyclophilin-catalysed peptidyl-prolyl isomerization promotes Aux/IAA degradation, as a mechanism regulating auxin signalling.

Suggested Citation

  • Hongwei Jing & Xiaolu Yang & Jian Zhang & Xuehui Liu & Huakun Zheng & Guojun Dong & Jinqiang Nian & Jian Feng & Bin Xia & Qian Qian & Jiayang Li & Jianru Zuo, 2015. "Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling," Nature Communications, Nature, vol. 6(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8395
    DOI: 10.1038/ncomms8395
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    Cited by:

    1. Veronika Řičařová & Jan Kazda & Khushwant Singh & Pavel Ryšánek, 2016. "Clubroot caused by Plasmodiophora brassicae Wor.: a review of emerging serious disease of oilseed rape in the Czech Republic," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 52(2), pages 71-86.
    2. Hongwei Jing & David A. Korasick & Ryan J. Emenecker & Nicholas Morffy & Edward G. Wilkinson & Samantha K. Powers & Lucia C. Strader, 2022. "Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Fuxi Rong & Yusong Lv & Pingchuan Deng & Xia Wu & Yaqi Zhang & Erkui Yue & Yuxin Shen & Sajid Muhammad & Fangrui Ni & Hongwu Bian & Xiangjin Wei & Weijun Zhou & Peisong Hu & Liang Wu, 2024. "Switching action modes of miR408-5p mediates auxin signaling in rice," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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