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Mitotic cell rounding and epithelial thinning regulate lumen growth and shape

Author

Listed:
  • Esteban Hoijman

    (Universitat Pompeu Fabra/PRBB)

  • Davide Rubbini

    (Universitat Pompeu Fabra/PRBB)

  • Julien Colombelli

    (Advanced Digital Microscopy, Institute for Research in Biomedicine)

  • Berta Alsina

    (Universitat Pompeu Fabra/PRBB)

Abstract

Many organ functions rely on epithelial cavities with particular shapes. Morphogenetic anomalies in these cavities lead to kidney, brain or inner ear diseases. Despite their relevance, the mechanisms regulating lumen dimensions are poorly understood. Here, we perform live imaging of zebrafish inner ear development and quantitatively analyse the dynamics of lumen growth in 3D. Using genetic, chemical and mechanical interferences, we identify two new morphogenetic mechanisms underlying anisotropic lumen growth. The first mechanism involves thinning of the epithelium as the cells change their shape and lose fluids in concert with expansion of the cavity, suggesting an intra-organ fluid redistribution process. In the second mechanism, revealed by laser microsurgery experiments, mitotic rounding cells apicobasally contract the epithelium and mechanically contribute to expansion of the lumen. Since these mechanisms are axis specific, they not only regulate lumen growth but also the shape of the cavity.

Suggested Citation

  • Esteban Hoijman & Davide Rubbini & Julien Colombelli & Berta Alsina, 2015. "Mitotic cell rounding and epithelial thinning regulate lumen growth and shape," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8355
    DOI: 10.1038/ncomms8355
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    Cited by:

    1. Sabrina Meindlhumer & Fridtjof Brauns & Jernej Rudi Finžgar & Jacob Kerssemakers & Cees Dekker & Erwin Frey, 2023. "Directing Min protein patterns with advective bulk flow," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Christophe Royer & Elizabeth Sandham & Elizabeth Slee & Falk Schneider & Christoffer B. Lagerholm & Jonathan Godwin & Nisha Veits & Holly Hathrell & Felix Zhou & Karolis Leonavicius & Jemma Garratt & , 2022. "ASPP2 maintains the integrity of mechanically stressed pseudostratified epithelia during morphogenesis," Nature Communications, Nature, vol. 13(1), pages 1-19, December.

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