IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8226.html
   My bibliography  Save this article

Maintenance of protein synthesis reading frame by EF-P and m1G37-tRNA

Author

Listed:
  • Howard B. Gamper

    (Thomas Jefferson University)

  • Isao Masuda

    (Thomas Jefferson University)

  • Milana Frenkel-Morgenstern

    (Faculty of Medicine, Bar-Ilan University)

  • Ya-Ming Hou

    (Thomas Jefferson University)

Abstract

Maintaining the translational reading frame poses difficulty for the ribosome. Slippery mRNA sequences such as CC[C/U]-[C/U], read by isoacceptors of tRNAPro, are highly prone to +1 frameshift (+1FS) errors. Here we show that +1FS errors occur by two mechanisms, a slow mechanism when tRNAPro is stalled in the P-site next to an empty A-site and a fast mechanism during translocation of tRNAPro into the P-site. Suppression of +1FS errors requires the m1G37 methylation of tRNAPro on the 3' side of the anticodon and the translation factor EF-P. Importantly, both m1G37 and EF-P show the strongest suppression effect when CC[C/U]-[C/U] are placed at the second codon of a reading frame. This work demonstrates that maintaining the reading frame immediately after the initiation of translation by the ribosome is an essential aspect of protein synthesis.

Suggested Citation

  • Howard B. Gamper & Isao Masuda & Milana Frenkel-Morgenstern & Ya-Ming Hou, 2015. "Maintenance of protein synthesis reading frame by EF-P and m1G37-tRNA," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8226
    DOI: 10.1038/ncomms8226
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8226
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8226?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8226. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.