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Optogenetic control of contractile function in skeletal muscle

Author

Listed:
  • Tobias Bruegmann

    (Institute of Physiology I, University of Bonn, Life and Brain Center
    Research Training Group 1873, University of Bonn)

  • Tobias van Bremen

    (University Hospital of Bonn)

  • Christoph C. Vogt

    (Institute of Physiology I, University of Bonn, Life and Brain Center)

  • Thorsten Send

    (University Hospital of Bonn)

  • Bernd K. Fleischmann

    (Institute of Physiology I, University of Bonn, Life and Brain Center)

  • Philipp Sasse

    (Institute of Physiology I, University of Bonn, Life and Brain Center)

Abstract

Optogenetic stimulation allows activation of cells with high spatial and temporal precision. Here we show direct optogenetic stimulation of skeletal muscle from transgenic mice expressing the light-sensitive channel Channelrhodopsin-2 (ChR2). Largest tetanic contractions are observed with 5-ms light pulses at 30 Hz, resulting in 84% of the maximal force induced by electrical stimulation. We demonstrate the utility of this approach by selectively stimulating with a light guide individual intralaryngeal muscles in explanted larynges from ChR2-transgenic mice, which enables selective opening and closing of the vocal cords. Furthermore, systemic injection of adeno-associated virus into wild-type mice provides sufficient ChR2 expression for optogenetic opening of the vocal cords. Thus, direct optogenetic stimulation of skeletal muscle generates large force and provides the distinct advantage of localized and cell-type-specific activation. This technology could be useful for therapeutic purposes, such as restoring the mobility of the vocal cords in patients suffering from laryngeal paralysis.

Suggested Citation

  • Tobias Bruegmann & Tobias van Bremen & Christoph C. Vogt & Thorsten Send & Bernd K. Fleischmann & Philipp Sasse, 2015. "Optogenetic control of contractile function in skeletal muscle," Nature Communications, Nature, vol. 6(1), pages 1-8, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8153
    DOI: 10.1038/ncomms8153
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    Cited by:

    1. Le Cai & Alex Burton & David A. Gonzales & Kevin Albert Kasper & Amirhossein Azami & Roberto Peralta & Megan Johnson & Jakob A. Bakall & Efren Barron Villalobos & Ethan C. Ross & John A. Szivek & Davi, 2021. "Osseosurface electronics—thin, wireless, battery-free and multimodal musculoskeletal biointerfaces," Nature Communications, Nature, vol. 12(1), pages 1-12, December.

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