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Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells

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  • Zhong-Wei Du

    (Waisman Center, University of Wisconsin)

  • Hong Chen

    (Waisman Center, University of Wisconsin
    Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology)

  • Huisheng Liu

    (Waisman Center, University of Wisconsin)

  • Jianfeng Lu

    (Waisman Center, University of Wisconsin)

  • Kun Qian

    (Waisman Center, University of Wisconsin
    Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology)

  • CindyTzu-Ling Huang

    (Waisman Center, University of Wisconsin)

  • Xiaofen Zhong

    (Waisman Center, University of Wisconsin)

  • Frank Fan

    (Promega Corporation)

  • Su-Chun Zhang

    (Waisman Center, University of Wisconsin
    School of Medicine and Public Health, University of Wisconsin)

Abstract

Human pluripotent stem cells (hPSCs) have opened new opportunities for understanding human development, modelling disease processes and developing new therapeutics. However, these applications are hindered by the low efficiency and heterogeneity of cell types, such as motorneurons (MNs), differentiated from hPSCs as well as our inability to maintain the potency of lineage-committed progenitors. Here by using a combination of small molecules that regulate multiple signalling pathways, we develop a method to guide human embryonic stem cells to a near-pure population (>95%) of motor neuron progenitors (MNPs) in 12 days, and an enriched population (>90%) of functionally mature MNs in an additional 16 days. More importantly, the MNPs can be expanded for at least five passages so that a single MNP can be amplified to 1 × 104. This method is reproducible in human-induced pluripotent stem cells and is applied to model MN-degenerative diseases and in proof-of-principle drug-screening assays.

Suggested Citation

  • Zhong-Wei Du & Hong Chen & Huisheng Liu & Jianfeng Lu & Kun Qian & CindyTzu-Ling Huang & Xiaofen Zhong & Frank Fan & Su-Chun Zhang, 2015. "Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7626
    DOI: 10.1038/ncomms7626
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    Cited by:

    1. Tao Wang & Xibin Tian & Han Byeol Kim & Yura Jang & Zhiyuan Huang & Chan Hyun Na & Jiou Wang, 2022. "Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules," Nature Communications, Nature, vol. 13(1), pages 1-21, December.

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