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Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation

Author

Listed:
  • Dawei Feng

    (Texas A&M University)

  • Tian-Fu Liu

    (Texas A&M University)

  • Jie Su

    (Berzelii Centre EXSELENT on Porous Materials and Inorganic and Structural Chemistry, Stockholm University)

  • Mathieu Bosch

    (Texas A&M University)

  • Zhangwen Wei

    (Texas A&M University)

  • Wei Wan

    (Berzelii Centre EXSELENT on Porous Materials and Inorganic and Structural Chemistry, Stockholm University)

  • Daqiang Yuan

    (State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fujian)

  • Ying-Pin Chen

    (Texas A&M University
    Texas A&M University)

  • Xuan Wang

    (Texas A&M University)

  • Kecheng Wang

    (Texas A&M University)

  • Xizhen Lian

    (Texas A&M University)

  • Zhi-Yuan Gu

    (Texas A&M University)

  • Jihye Park

    (Texas A&M University)

  • Xiaodong Zou

    (Berzelii Centre EXSELENT on Porous Materials and Inorganic and Structural Chemistry, Stockholm University)

  • Hong-Cai Zhou

    (Texas A&M University
    Texas A&M University)

Abstract

Enzymatic catalytic processes possess great potential in chemical manufacturing, including pharmaceuticals, fuel production and food processing. However, the engineering of enzymes is severely hampered due to their low operational stability and difficulty of reuse. Here, we develop a series of stable metal-organic frameworks with rationally designed ultra-large mesoporous cages as single-molecule traps (SMTs) for enzyme encapsulation. With a high concentration of mesoporous cages as SMTs, PCN-333(Al) encapsulates three enzymes with record-high loadings and recyclability. Immobilized enzymes that most likely undergo single-enzyme encapsulation (SEE) show smaller Km than free enzymes while maintaining comparable catalytic efficiency. Under harsh conditions, the enzyme in SEE exhibits better performance than free enzyme, showing the effectiveness of SEE in preventing enzyme aggregation or denaturation. With extraordinarily large pore size and excellent chemical stability, PCN-333 may be of interest not only for enzyme encapsulation, but also for entrapment of other nanoscaled functional moieties.

Suggested Citation

  • Dawei Feng & Tian-Fu Liu & Jie Su & Mathieu Bosch & Zhangwen Wei & Wei Wan & Daqiang Yuan & Ying-Pin Chen & Xuan Wang & Kecheng Wang & Xizhen Lian & Zhi-Yuan Gu & Jihye Park & Xiaodong Zou & Hong-Cai , 2015. "Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation," Nature Communications, Nature, vol. 6(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6979
    DOI: 10.1038/ncomms6979
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    Cited by:

    1. Yu Liang & Xiaoxin Yang & Xiaoyu Wang & Zong-Jie Guan & Hang Xing & Yu Fang, 2023. "A cage-on-MOF strategy to coordinatively functionalize mesoporous MOFs for manipulating selectivity in adsorption and catalysis," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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