Author
Listed:
- Gijs van der Schot
(Laboratory of Molecular Biophysics, Uppsala University)
- Martin Svenda
(Laboratory of Molecular Biophysics, Uppsala University)
- Filipe R. N. C. Maia
(Laboratory of Molecular Biophysics, Uppsala University
Lawrence Berkeley National Lab, 1 Cyclotron Road Mail Stop 943–256)
- Max Hantke
(Laboratory of Molecular Biophysics, Uppsala University)
- Daniel P. DePonte
(Center for Free-Electron Laser Science, DESY
LCLS, SLAC National Accelerator Laboratory)
- M. Marvin Seibert
(Laboratory of Molecular Biophysics, Uppsala University
LCLS, SLAC National Accelerator Laboratory)
- Andrew Aquila
(Center for Free-Electron Laser Science, DESY
The European XFEL GmbH)
- Joachim Schulz
(Center for Free-Electron Laser Science, DESY
The European XFEL GmbH)
- Richard Kirian
(Center for Free-Electron Laser Science, DESY)
- Mengning Liang
(Center for Free-Electron Laser Science, DESY)
- Francesco Stellato
(Center for Free-Electron Laser Science, DESY
University of Rome ‘Tor Vergata’)
- Bianca Iwan
(Laboratory of Molecular Biophysics, Uppsala University)
- Jakob Andreasson
(Laboratory of Molecular Biophysics, Uppsala University)
- Nicusor Timneanu
(Laboratory of Molecular Biophysics, Uppsala University)
- Daniel Westphal
(Laboratory of Molecular Biophysics, Uppsala University)
- F. Nunes Almeida
(Laboratory of Molecular Biophysics, Uppsala University)
- Dusko Odic
(Laboratory of Molecular Biophysics, Uppsala University)
- Dirk Hasse
(Laboratory of Molecular Biophysics, Uppsala University)
- Gunilla H. Carlsson
(Laboratory of Molecular Biophysics, Uppsala University)
- Daniel S. D. Larsson
(Laboratory of Molecular Biophysics, Uppsala University)
- Anton Barty
(Lawrence Berkeley National Lab, 1 Cyclotron Road Mail Stop 943–256)
- Andrew V. Martin
(Center for Free-Electron Laser Science, DESY
ARC Centre of Excellence for Coherent X-ray Science, School of Physics, University of Melbourne)
- Sebastian Schorb
(LCLS, SLAC National Accelerator Laboratory)
- Christoph Bostedt
(LCLS, SLAC National Accelerator Laboratory)
- John D. Bozek
(LCLS, SLAC National Accelerator Laboratory)
- Daniel Rolles
(Center for Free-Electron Laser Science, DESY)
- Artem Rudenko
(Center for Free-Electron Laser Science, DESY
Kansas State University)
- Sascha Epp
(Center for Free-Electron Laser Science, DESY)
- Lutz Foucar
(Max-Planck-Institut für medizinische Forschung)
- Benedikt Rudek
(Physikalisch-Technische Bundesanstalt (PTB) Bundesallee 100)
- Robert Hartmann
(PNSensor GmbH)
- Nils Kimmel
(PNSensor GmbH
Max Planck Institute for Extraterrestrial Physics, Giessenbachstrasse)
- Peter Holl
(PNSensor GmbH)
- Lars Englert
(Institute of Physics, Carl von Ossietzky Universitaet Oldenburg, Carl-von-Ossietzky-Straße 9-11)
- Ne-Te Duane Loh
(Centre for BioImaging Sciences, National University of Singapore)
- Henry N. Chapman
(Center for Free-Electron Laser Science, DESY
University of Hamburg)
- Inger Andersson
(Laboratory of Molecular Biophysics, Uppsala University)
- Janos Hajdu
(Laboratory of Molecular Biophysics, Uppsala University
The European XFEL GmbH)
- Tomas Ekeberg
(Laboratory of Molecular Biophysics, Uppsala University)
Abstract
There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.
Suggested Citation
Gijs van der Schot & Martin Svenda & Filipe R. N. C. Maia & Max Hantke & Daniel P. DePonte & M. Marvin Seibert & Andrew Aquila & Joachim Schulz & Richard Kirian & Mengning Liang & Francesco Stellato &, 2015.
"Imaging single cells in a beam of live cyanobacteria with an X-ray laser,"
Nature Communications, Nature, vol. 6(1), pages 1-9, May.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6704
DOI: 10.1038/ncomms6704
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