IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms6704.html
   My bibliography  Save this article

Imaging single cells in a beam of live cyanobacteria with an X-ray laser

Author

Listed:
  • Gijs van der Schot

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Martin Svenda

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Filipe R. N. C. Maia

    (Laboratory of Molecular Biophysics, Uppsala University
    Lawrence Berkeley National Lab, 1 Cyclotron Road Mail Stop 943–256)

  • Max Hantke

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Daniel P. DePonte

    (Center for Free-Electron Laser Science, DESY
    LCLS, SLAC National Accelerator Laboratory)

  • M. Marvin Seibert

    (Laboratory of Molecular Biophysics, Uppsala University
    LCLS, SLAC National Accelerator Laboratory)

  • Andrew Aquila

    (Center for Free-Electron Laser Science, DESY
    The European XFEL GmbH)

  • Joachim Schulz

    (Center for Free-Electron Laser Science, DESY
    The European XFEL GmbH)

  • Richard Kirian

    (Center for Free-Electron Laser Science, DESY)

  • Mengning Liang

    (Center for Free-Electron Laser Science, DESY)

  • Francesco Stellato

    (Center for Free-Electron Laser Science, DESY
    University of Rome ‘Tor Vergata’)

  • Bianca Iwan

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Jakob Andreasson

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Nicusor Timneanu

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Daniel Westphal

    (Laboratory of Molecular Biophysics, Uppsala University)

  • F. Nunes Almeida

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Dusko Odic

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Dirk Hasse

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Gunilla H. Carlsson

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Daniel S. D. Larsson

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Anton Barty

    (Lawrence Berkeley National Lab, 1 Cyclotron Road Mail Stop 943–256)

  • Andrew V. Martin

    (Center for Free-Electron Laser Science, DESY
    ARC Centre of Excellence for Coherent X-ray Science, School of Physics, University of Melbourne)

  • Sebastian Schorb

    (LCLS, SLAC National Accelerator Laboratory)

  • Christoph Bostedt

    (LCLS, SLAC National Accelerator Laboratory)

  • John D. Bozek

    (LCLS, SLAC National Accelerator Laboratory)

  • Daniel Rolles

    (Center for Free-Electron Laser Science, DESY)

  • Artem Rudenko

    (Center for Free-Electron Laser Science, DESY
    Kansas State University)

  • Sascha Epp

    (Center for Free-Electron Laser Science, DESY)

  • Lutz Foucar

    (Max-Planck-Institut für medizinische Forschung)

  • Benedikt Rudek

    (Physikalisch-Technische Bundesanstalt (PTB) Bundesallee 100)

  • Robert Hartmann

    (PNSensor GmbH)

  • Nils Kimmel

    (PNSensor GmbH
    Max Planck Institute for Extraterrestrial Physics, Giessenbachstrasse)

  • Peter Holl

    (PNSensor GmbH)

  • Lars Englert

    (Institute of Physics, Carl von Ossietzky Universitaet Oldenburg, Carl-von-Ossietzky-Straße 9-11)

  • Ne-Te Duane Loh

    (Centre for BioImaging Sciences, National University of Singapore)

  • Henry N. Chapman

    (Center for Free-Electron Laser Science, DESY
    University of Hamburg)

  • Inger Andersson

    (Laboratory of Molecular Biophysics, Uppsala University)

  • Janos Hajdu

    (Laboratory of Molecular Biophysics, Uppsala University
    The European XFEL GmbH)

  • Tomas Ekeberg

    (Laboratory of Molecular Biophysics, Uppsala University)

Abstract

There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.

Suggested Citation

  • Gijs van der Schot & Martin Svenda & Filipe R. N. C. Maia & Max Hantke & Daniel P. DePonte & M. Marvin Seibert & Andrew Aquila & Joachim Schulz & Richard Kirian & Mengning Liang & Francesco Stellato &, 2015. "Imaging single cells in a beam of live cyanobacteria with an X-ray laser," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
  • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6704
    DOI: 10.1038/ncomms6704
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms6704
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/ncomms6704?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hirokatsu Yumoto & Takahisa Koyama & Akihiro Suzuki & Yasumasa Joti & Yoshiya Niida & Kensuke Tono & Yoshitaka Bessho & Makina Yabashi & Yoshinori Nishino & Haruhiko Ohashi, 2022. "High-fluence and high-gain multilayer focusing optics to enhance spatial resolution in femtosecond X-ray laser imaging," Nature Communications, Nature, vol. 13(1), pages 1-8, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6704. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.