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Programmable biofilm-based materials from engineered curli nanofibres

Author

Listed:
  • Peter Q. Nguyen

    (School of Engineering and Applied Sciences, Harvard University
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

  • Zsofia Botyanszki

    (Wyss Institute for Biologically Inspired Engineering, Harvard University
    Harvard University)

  • Pei Kun R. Tay

    (School of Engineering and Applied Sciences, Harvard University
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

  • Neel S. Joshi

    (School of Engineering and Applied Sciences, Harvard University
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

Abstract

The significant role of biofilms in pathogenicity has spurred research into preventing their formation and promoting their disruption, resulting in overlooked opportunities to develop biofilms as a synthetic biological platform for self-assembling functional materials. Here we present Biofilm-Integrated Nanofiber Display (BIND) as a strategy for the molecular programming of the bacterial extracellular matrix material by genetically appending peptide domains to the amyloid protein CsgA, the dominant proteinaceous component in Escherichia coli biofilms. These engineered CsgA fusion proteins are successfully secreted and extracellularly self-assemble into amyloid nanofibre networks that retain the functions of the displayed peptide domains. We show the use of BIND to confer diverse artificial functions to the biofilm matrix, such as nanoparticle biotemplating, substrate adhesion, covalent immobilization of proteins or a combination thereof. BIND is a versatile nanobiotechnological platform for developing robust materials with programmable functions, demonstrating the potential of utilizing biofilms as large-scale designable biomaterials.

Suggested Citation

  • Peter Q. Nguyen & Zsofia Botyanszki & Pei Kun R. Tay & Neel S. Joshi, 2014. "Programmable biofilm-based materials from engineered curli nanofibres," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5945
    DOI: 10.1038/ncomms5945
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    Cited by:

    1. Anna M. Duraj-Thatte & Avinash Manjula-Basavanna & Jarod Rutledge & Jing Xia & Shabir Hassan & Arjirios Sourlis & Andrés G. Rubio & Ami Lesha & Michael Zenkl & Anton Kan & David A. Weitz & Yu Shrike Z, 2021. "Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Mike Sleutel & Brajabandhu Pradhan & Alexander N. Volkov & Han Remaut, 2023. "Structural analysis and architectural principles of the bacterial amyloid curli," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Avinash Manjula-Basavanna & Anna M. Duraj-Thatte & Neel S. Joshi, 2024. "Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    4. Sun-Young Kang & Anaya Pokhrel & Sara Bratsch & Joey J. Benson & Seung-Oh Seo & Maureen B. Quin & Alptekin Aksan & Claudia Schmidt-Dannert, 2021. "Engineering Bacillus subtilis for the formation of a durable living biocomposite material," Nature Communications, Nature, vol. 12(1), pages 1-17, December.

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