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Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells

Author

Listed:
  • Xinwei Yu

    (Boston University)

  • Amin Feizpour

    (Boston University)

  • Nora-Guadalupe P. Ramirez

    (Boston University School of Medicine)

  • Linxi Wu

    (Boston University)

  • Hisashi Akiyama

    (Boston University School of Medicine)

  • Fangda Xu

    (Boston University)

  • Suryaram Gummuluru

    (Boston University School of Medicine)

  • Björn M. Reinhard

    (Boston University)

Abstract

Ganglioside GM3, a host-derived glycosphingolipid incorporated in the membrane of human immunodeficiency virus-1 (HIV-1) viral particles, mediates interactions between HIV-1 and Siglec1/CD169, a protein expressed on dendritic cells (DCs). Such interactions, which seem to be independent of viral envelope glycoprotein gp120, are poorly understood. Here we develop a model system consisting of self-assembled artificial virus nanoparticles (AVNs) that are free of viral glycoproteins or other host-derived glycolipids and glycoproteins. These plasmonic AVNs contain a membrane of defined composition wrapped around a solid metal core. GM3-containing AVNs are captured by CD169-expressing HeLa cells or mature DCs, and are sequestered within non-lysosomal tetraspanin-positive compartments. This distribution is reminiscent of CD169-dependent HIV-1 sequestration in mature DCs. Our results highlight GM3–CD169 binding as a gp120-independent signal for sequestration and preservation of HIV-1 infectivity. They also indicate that plasmonic AVNs offer improved features over liposome-based systems and represent a versatile tool for probing specific virus–cell interactions.

Suggested Citation

  • Xinwei Yu & Amin Feizpour & Nora-Guadalupe P. Ramirez & Linxi Wu & Hisashi Akiyama & Fangda Xu & Suryaram Gummuluru & Björn M. Reinhard, 2014. "Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells," Nature Communications, Nature, vol. 5(1), pages 1-12, September.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5136
    DOI: 10.1038/ncomms5136
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    Cited by:

    1. Muhammad Yaseen & Muhammad Humayun & Abbas Khan & Muhammad Usman & Habib Ullah & Asif Ali Tahir & Habib Ullah, 2021. "Preparation, Functionalization, Modification, and Applications of Nanostructured Gold: A Critical Review," Energies, MDPI, vol. 14(5), pages 1-88, February.

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