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Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Author

Listed:
  • Yusuke Yoshioka

    (National Cancer Center Research Institute
    Integrative Bioscience and Biomedical Engineering, Graduate School of Advanced Science and Engineering, Waseda University
    Research Fellow of the Japan Society for the Promotion of Science (JSPS))

  • Nobuyoshi Kosaka

    (National Cancer Center Research Institute)

  • Yuki Konishi

    (National Cancer Center Research Institute
    SRL Inc.)

  • Hideki Ohta

    (Shionogi & Co., LTD.)

  • Hiroyuki Okamoto

    (Shionogi & Co., LTD.)

  • Hikaru Sonoda

    (Shionogi & Co., LTD.)

  • Ryoji Nonaka

    (Graduated School of Medicine, Osaka University)

  • Hirofumi Yamamoto

    (Graduated School of Medicine, Osaka University)

  • Hideshi Ishii

    (Osaka University, Graduate School of Medicine)

  • Masaki Mori

    (Graduated School of Medicine, Osaka University)

  • Koh Furuta

    (National Cancer Center Hospital)

  • Takeshi Nakajima

    (National Cancer Center Hospital)

  • Hiroshi Hayashi

    (SRL Inc.)

  • Hajime Sugisaki

    (SRL Inc.)

  • Hiroko Higashimoto

    (SRL Inc.)

  • Takashi Kato

    (Integrative Bioscience and Biomedical Engineering, Graduate School of Advanced Science and Engineering, Waseda University)

  • Fumitaka Takeshita

    (National Cancer Center Research Institute)

  • Takahiro Ochiya

    (National Cancer Center Research Institute)

Abstract

Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

Suggested Citation

  • Yusuke Yoshioka & Nobuyoshi Kosaka & Yuki Konishi & Hideki Ohta & Hiroyuki Okamoto & Hikaru Sonoda & Ryoji Nonaka & Hirofumi Yamamoto & Hideshi Ishii & Masaki Mori & Koh Furuta & Takeshi Nakajima & Hi, 2014. "Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4591
    DOI: 10.1038/ncomms4591
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    Cited by:

    1. Yue Jiang & Min Zhao & Jia Miao & Wan Chen & Yuan Zhang & Minqian Miao & Li Yang & Qing Li & Qingqing Miao, 2024. "Acidity-activatable upconversion afterglow luminescence cocktail nanoparticles for ultrasensitive in vivo imaging," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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